NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Tularemia PCR test for dogs and cats

dog and cat assay data sheet

Tularemia (Francisella tularensis)

Test code: B0058 - Ultrasensitive qualitative detection of Francisella tularensis by real time polymerase chain reaction.

B0058 is included on P0025 - tickborne disease panel

Francisella tularensis is a small, Gram-negative, aerobic bacillus with two main serotypes: Jellison Type A and Type B. Type A is the more virulent form. Tularemia is frequently spread by direct contact with rabbits, leading to the term "rabbit fever." The disease, however, can also be spread by other animals, such as rodents, and by ticks.

F. tularensis is very resistant to environmental changes and is capable of surviving for weeks at low temperatures in water, moist soil, hay, straw, or decaying animal carcasses. Small mammals such as voles, mice, water rats, squirrels, rabbits and hares are natural reservoirs for F. tularensis. These animals become infected by the bacteria through bites from ticks, fleas, and mosquitoes and contact with contaminated environments. People are infected through bites from infected arthropods (usually ticks), contact with infected animal tissues or fluids, direct contact with or ingestion of contaminated water, food, or soil, or inhalation of aerosolized bacteria. F. tularensis is very infectious so that the simple act of examining an open laboratory culture plate without adequate protective equipment can lead to infection and disease. For this reason, F. tularensis is classified as a bioterrorism agent and culture detection of the bacteria without suitable laboratory facilities is not recommended.

People infected by F. tularensis can develop symptoms in about 3-5 days, but some people may take as long as two weeks for symptoms to develop. Symptoms vary based on mode of infection, but generally include fever, chills, joint and muscle pain, headache, weakness, and sometimes pneumonia. People who develop pneumonic tularemia experience chest pain, bloody sputum and difficulty breathing. The disease is easily cured by antibiotic treatment.

Culture detection of the bacteria is usually not suitable due to the highly infectious nature of the bacteria, and because culture detection is not very sensitive (Johansson et al., 2000). Diagnosis based on serology is not reliable because some infected people may not develop antibodies (Johansson et al., 2000). Molecular detection of the bacteria is a rapid, sensitive and specific method for confirming F. tularensis infection.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Tularemia infection
  • Help ensure that animal populations are free of Tularemia
  • Early prevention of the spread of Tularemia
  • Minimize human exposure to Tularemia
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Johansson, A., Berglund, L., Eriksson, U., Göransson, I., Wollin, R., Forsman, M., Tärnvik, A. and Sjöstedt, A. (2000) Comparative Analysis of PCR versus Culture for Diagnosis of Ulceroglandular Tularemia. J. Clin. Microbiol. 38: 22-26.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml synovial fluid, or tick.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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