Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature for several weeks.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Aspergillus

Babesia

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Anaplasma phagocytophilum PCR test

equine assay data sheet

Anaplasma phagocytophilum

Test code:
B0101 - Ultrasensitive detection of Anaplasma phagocytophilum (formerly Ehrlichia phagocytophilum) by real time PCR

 

Anaplasma phagocytophilum is a gram-negative bacterium formerly known as Ehrlichia phagocytophilum. It causes anaplasmosis in horses, and also in sheep and cattle, where it is known as tick-borne fever and pasture fever respectively. The bacteria tend to attack neutrophils and when they infect humans, they can cause human granulocytic anaplasmosis.

The bacteria are transmitted to humans by the bite of an infected tick. The black-legged tick (Ixodes scapularis) is the vector of A. phagocytophilum in the northeastern and upper midwestern United States. The western black-legged tick (Ixodes pacificus) is the primary vector in Northern California.

When humans are infected, it may take one to two weeks before symptoms develop. Symptoms include fever, headache, muscle pain, skin rash (in rare occasions), leukopenia, thrombocytopenia and mild injury to the liver.

When animals are infected with these bacteria, they may develop lethargy, ataxia, inappetence, and weak or painful limbs, but the most severe changes are anemia and leukopenia. The infection is often confused with Lyme disease, another tick-borne illness.

Serological detection of these bacteria can take some time to reach a definitive diagnosis. Molecular detection by polymerase chain reaction (PCR) is a highly specific and sensitive way to detect the presence of these bacteria, and should be considered as an alternative to the serological method (Kirtz et al., 2005).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Anaplasma infection
  • Help ensure that herds are free of Anaplasma
  • Early prevention of spread of Anaplasma among animals
  • Minimize human exposure to Anaplasma
  • Safety monitoring of biological products that derive from susceptible animals.

References:
Kirtz, G., Meli, M., Leidinger, E., Ludwig, P., Thum, D., Czettel, B., Kölbl, S. and Lutz, H. (2005) Anaplasma phagocytophilum infection in a dog: identifying the causative agent using PCR. J. Small Anim. Pract. 46:300-303.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

African horse sickness PCR test

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