Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature for several weeks.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Aspergillus

Babesia

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Equine viral arteritis PCR test

equine assay data sheet

Equine arteritis virus (EAV)

Test code:
S0066 - Ultrasensitive qualitative detection of equine arteritis virus by reverse transcription coupled real time polymerase chain reaction. This assay detects but does not differentiate most known EAV strains.

S0066 is included in P0013 - equine respiratory panel and in P0024 - equine breeding panel

 

Equine arteritis virus (EAV) is a positive stranded RNA virus in the family Arteriviridae, order Nidovirales, and infects horses worldwide. Sporadic respiratory disease and sudden death in foals, abortion in mares, and mild or subclinical infections in adult horses (Del Piero, 2000) have been described resulting from this infection. Adult stallions may become chronically infected; they can become a reservoir and spread the virus via their semen (Timoney and McCollum, 1993).

Clinical signs of EAV infection include depression, anorexia, jaundice, rash, nasal discharge, cough, swelling of the lower limbs, scrotum or mammary gland, periobital swelling and conjunctivitis (pink eye). Mares and geldings eliminate infection within 21 to 30 days. However, 30-60 % of infected stallions become carrriers and shed the virus in their semen.

Only one serotype of EAV is reported, but there are several strains that differ in their virulence and neutralization phenotype. Novel genetic variants of EAV can arise during persistent infection of stallions, and the continued movement of persistently infected stallions and infective semen have created a requirement for rapid, sensitive and specific tests to detect EAV.

The presence of EAV in the semen of carrier stallions can be detected by virus isolation in cell culture or by test breeding of stallions to susceptible mares but both techniques are time consuming, expensive and cumbersome (Timoney and McCollum, 1993; Mann, 1997,1998). Serologic detection of this virus is not reliable since the serologic response of individual horses to EAV may vary with the infecting virus strain and duration of infection (MacLachlan et al., 1998). Molecular detection of EAV is a fast, specific and reliable method for determining EAV infection status.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of EAV infection.
  • Help ensure that horse populations are free of EAV
  • Early prevention of spread of this virus
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from horses

References:
Del Piero, F. (2000) Equine Viral Arteritis, review article. Vet. Pathol. 37:287-296.
MacLachlan, N.J., Balasuriya, U.B., Hedges, J.F., Schweidler, T.M., McCollum, W.H., Timoney, P.J., Hullinger, P.J. and Patton, J.F. (1998) Serologic Response of Horses to the Structural Proteins of Equine Arteritis Virus. J Vet Diagn Invest. 10:229-36.
Mann, A.W. (1997) Addressing Equine Viral Arteritis in the United States. Proc. Annu. Meet. U.S. Anim. Health Assoc., pp. 259–264.
Mann, A.W. (1998) Equine Viral Arteritis. Proc. Annu. Meet. U.S. Anim. Health Assoc., pp. 314–316.
Timoney, P. J. and W. H. McCollum (1993) Equine Viral Arteritis. Vet. Clin. N. Am. Equine Pract. 9:295-309.

Specimen requirements: Nasopharyngeal swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml semen, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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