Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature for several weeks.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Aspergillus

Babesia

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Equine herpes 2 PCR test

equine assay data sheet

Equine herpesvirus type II (EHV-2), formerly "equine cytomegalovirus"

Test code:
S0072 - Ultrasensitive qualitative detection of equine herpesvirus type II by real time polymerase chain reaction

 

Equine herpesvirus type II (EHV-2) is a DNA virus belonging to the gammaherpesvirus family. This virus infects B cells and is related to other lymphotropic herpesviruses such as Epstein-Barr virus (EBV, which causes glandular fever) and human herpesvirus type 8 (HHV-8), also known as Kaposi's sarcoma herpesvirus.  Equine herpesvirus II was previously known as "equine CMV (cytomegalovirus)".

EHV-2 is highly prevalent (approaching 100%) in horse populations (Borchers et al., 1999) and has been proposed to cause, or to be a predisposing factor for, a number of distinct syndromes. These include “loss of performance”, conjunctivitis (Kershaw et al., 2001) and respiratory tract disease. EHV-2 has also been suggested as a possible transactivating factor triggering or exacerbating EHV-1 or EHV-4 reactivation and disease. While the association of EHV-2 with particular diseases is subject to debate, it is clear that this virus has the potential to escape and subvert host immune responses.

Serological detection of EHV-2 is of limited use to determine the re-activation of this herpes virus. EHV-2 can remain latent in affected animals while shedding at levels adequate to infect other horses. Culture identification of EHV-2 is slow and has low sensitivity. Molecular detection of EHV-2 by PCR is the most sensitive, specific and accurate tool in assessing the infectivity of an affected horse.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of EHV-2 infection
  • Help ensure that horse populations are free of EHV-2
  • Early prevention of spread of this virus
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from horses

References:
Slater, J. D., Borchers, K., Thackray, A. M. and Field, H. J. (1994) The trigeminal ganglion is a location for equine herpesvirus 1 latency and reactivation in the horse. J. Gen. Virol. 75: 2007–2016.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasopharyngeal swab, or 0.2 ml broncheoalveolar lavage, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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