Respiratory symptoms got you breathless? Try our equine respiratory PCR panel -- we test for 7 respiratory bacteria and viruses from 1 swab.

Neurological symptoms got you down? Try our equine neurological PCR panel -- we test for 5 neurological diseases from 1 CSF or tissue sample.

Diarrhea got you on the run? Try our equine GI / diarrhea PCR panel -- we test for 4 GI diseases from 1 fecal or swab sample.

Oh baby! Our equine breeding PCR panel tests for 5 diseases affecting breeding success from 1 swab or semen sample.

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For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature for several weeks.

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Zoologix performs equine PCR tests for...

African horse sickness

Anaplasma phagocytophilum

Aspergillus

Babesia

Borrelia burgdorferi

Burkholderia mallei and pseudomallei

Clostridium difficile

Clostridium species

Contagious equine metritis (CEM)

Coronaviruses

Dourine

Eastern equine encephalitis (EEE)

E. coli O157:H7

E. coli panel

Equine adenoviruses

Equine arteritis virus (EAV)

Equine herpesvirus
type 1

Equine herpesvirus
type 2

Equine herpesvirus
type 3

Equine herpesvirus
type 4

Equine herpesvirus
type 5

Equine infectious anemia (EIA)

Equine piroplasmosis

Equine protozoal myeloencephalitis (EPM)

Giardia

Glanders

Helicobacter

Histoplasma

Horsepox virus

Influenza

Japanese encephalitis

Lawsonia intracellularis

Leptospirosis

Lyme disease

Melioidosis

Neospora caninum

Neospora hughesi

Piroplasmosis

Potomac horse fever

Rabies

Rhodococcus equi

Rotavirus

Sarcocystis neurona

St. Louis encephalitis

Strangles (Strep equi)

Streptococcus pneumoniae

Strongyles

Surra

Tapeworms

Taylorella equigenitalis

Theileria equi

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma equiperdum

Trypanosoma evansi

Venezuelan equine encephalitis (VEE)

Vesicular stomatitis

West Nile virus (WNV)

Western Equine Encephalitis (WEE)

Yersinia enterocolitica

Yersinia pseudotuberculosis

Genetic tests for...

Hyperkalemic periodic paralysis


Lawsonia PCR test for horses
equine assay data sheet

Lawsonia intracellularis

Test code:
B0035 - Ultrasensitive qualitative detection of Lawsonia intracellularis by real time polymerase chain reaction.

B0035 is included in P0015 - equine GI/diarrhea panel

 

Proliferative enteropathy, also known as proliferative ileitis, is caused by infection with Lawsonia intracellularis, an obligate intracellular, curve-shaped, argyrophilic bacterium. The disease has been detected in domestic and laboratory animals including primates, pig, horse, dog, rat, ferret, guinea pig, rabbit and hamster. The disease has been reported sporadically in foals 3-7 months of age and one outbreak involving 3 different breeding farms has been described. All reported cases were in the eastern half of Canada or the United States. The swine industry suffers the most significant impact from this disease. The disease has two clinical manifestations in pigs: an acute hemorrhagic form often called porcine hemorrhagic enteropathy, and a more chronic proliferative form often referred as porcine intestinal adenomatosis.

Environmental contamination with feces of infected animals appears to be the most important route of transmission of disease, but it is currently unknown how long Lawsonia intracellularis can remain infectious outside the animal. Infection occurs most often during the post-weaning period, when passive maternal immunity declines. After ingestion, the bacteria infect intestinal proliferating crypt epithelial cells and multiply within the apical cytoplasm. There is no evidence of infection of tissues other than intestine. Most infected animals have subclinical infection but shed the bacteria in their feces, leading to environmental contamination. Clinical manifestation of an infection can be triggered by stressors, such as overcrowding, transport, change in diet, and experimental manipulation.

Bacterial culture and isolation of the bacteria are difficult because cultured enterocytes are required to support the growth of Lawsonia intracellularis. Electron microscopy can be used to detect the curved bacterial rods in apical cytoplasm of enterocytes but this is a very time-consuming process and few laboratories have such capability. Immunohistochemical detection can be performed on formalin-fixed, paraffin-embedded tissue from affected intestine to detect the bacteria in following biopsy or necropsy, but this is also a very time-consuming process. Molecular detection using PCR is the most sensitive, rapid and specific method to confirm presence of the Lawsonia intracellularis genome within tissue samples (Jones et al., 1993).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Lawsonia infection.
  • Help ensure that horse populations are free of Lawsonia
  • Early prevention of spread of this bacterium
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products that derive from horses

References:
Jones, G. F., Ward, G.E., Murtaugh, M.P., Lin, G. and Gebhart, C.J. (1993) Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis in feces by polymerase chain reaction. J. Clin. Microbiol. 31:2611-2615.

Specimen requirements: 0.2 ml feces, or rectal swab, or 0.2 ml fresh, frozen or fixed ileum tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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