- Ultrasensitive qualitative detection of Neisseria
gonorrhoeae by real time polymerase chain reaction.
- Chlamydia trachomatis and Neisseria gonorhoeae
screen by real time polymerase chain reaction. This screen
detects but does not differentiate N. gonorhoeae and
Neisseria gonorrhoeae are gram-negative diplococcus bacteria. Although
humans are the only natural host for these bacteria, other
species have been infected experimentally, including guinea
pigs, rabbits, mice, hamsters, rats, rhesus monkeys,
chimpanzees, and baboons.
In humans, infection with these bacteria causes gonorrhea, a
sexually transmitted disease of worldwide significance. The
highest attack rate in both men and women occurs between 15 and
29 years of age. Host-related factors such as the number of
sexual partners, contraceptive practices, sexual preference, and
population mobility contribute to the incidence of gonorrhea.
N. gonorrhoeae can
grow and multiply easily in warm, moist areas, including the
cervix, uterus, and fallopian tubes in women, and in the urethra
in women and men. The bacteria can also grow in the mouth,
throat, eyes, and anus.
These bacteria adhere to columnar epithelial cells, penetrate
them, then multiply on the basement membrane. Adherence is
facilitated through pili and opa proteins. Infection with these
bacteria can result in urethritis, cervicitis, salpingitis,
pelvic inflammatory disease, proctitis, conjunctivitis and
pharyngitis. Transmission occurs through direct contact.
Detection of N.
gonorrhoeae by PCR is highly sensitivity and specific (Luijt
et al., 2005).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of
Neisseria gonorrhoeae infection.
Help ensure that colonies are free of N. gonorrhoeae
Early prevention of spread of N. gonorrhoeae
Minimize personnel exposure to N. gonorrhoeae
Safety monitoring of biological products
Luijt, D.S., Bos,
P.A.J., van Zwet, A.A., van Voorst Vader, P.C. and Schirm,
J. (2005) Comparison of COBAS AMPLICOR Neisseria gonorrhoeae
PCR, including Confirmation with N. gonorrhoeae-specific
16S rRNA PCR, with traditional culture.
J. Clin. Microbiol. 43:1445-1447
0.2 ml urine, or
urogenital swab, or vaginal swab, or eye swab, or 0.2 ml
For specimen types other than those listed here, please call to
confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Qualitative real time PCR
Normal range: Nondetected