Simian hemorrhagic fever virus (SHFV)
S0113 - Ultrasensitive
qualitative detection of simian hemorrhagic
fever virus by reverse transcription coupled real time PCR
hemorrhagic fever virus (SHFV) was first isolated in 1964 from
rhesus macaques with hemorrhagic disease in research colonies in
the United States and Russia.
SHFV is a
positive-strand RNA virus that was recently classified in the
family Arteriviridae along with equine arteritis virus (EAV),
lactate dehydrogenase-elevating virus (LDV), and porcine
reproductive and respiratory syndrome virus (PRRSV), within the
new order Nidovirales.
infection of SHFV can occur in patas monkeys, vervet monkeys,
and baboons. Patas monkeys are thought to be the natural host
for the virus. About 50% of wild patas monkeys have antibodies,
while antibodies are much less prevalent in vervets and baboons.
It is estimated that approximately 10% of patas monkeys may be
asymptomatic carriers. In macaques (M.
mulatta, M. fascicularis, M. arctoides, M. assamensis, M.
nemestrina) infection with this virus can result in
acute severe disease with high mortality.
with this virus has a rapid onset. Animals develop fever, facial
edema, cyanosis, anorexia, melena, and may have cutaneous,
subcutaneous, and retrobulbar hemorrhage. Thrombocytopenia will
also develop. The clinical course typically lasts for 10-15 days
tests cannot distinguish carriers from previously infected
animals, and virus isolation is generally unreliable for
diagnosis. Molecular detection by PCR has become the method of
choice for detection of simian hemorrhagic fever.
Help confirm the disease causing agent
Identify carriers of SHFV
Help ensure that animal colonies are free of SHFV
Early prevention of spread of this virus among a colony
Minimize unnecessary occupational exposure to this virus
Safety monitoring of biological products and vaccines
that derive from monkeys
Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
reverse transcription coupled real time polymerase chain