Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

For wild rodent infestations, remediation and environmental monitoring, use our Rodent Infestation PCR Panel

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Zoologix also performs rodent and rabbit PCR tests for...

Aspiculuris tetraptera

Bordetella

BXV-1 virus

Campylobacter

Chapparvovirus

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalitozoon cuniculi

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Leptospira

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse kidney parvovirus (MKV or MKPV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit coronavirus

Rabbit fibroma virus

Rabbit hemorrhagic disease virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi/ paraluiscuniculi

Tularemia

Tyzzer's disease

Whitewater Arroyo virus

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Campylobacter PCR test for rodents
rodent and rabbit assay data sheet

Campylobacter

Test codes:

B0006 - Qualitative Campylobacter species screen by real time polymerase chain reaction. This assay detects but does not differentiate C. jejuni, C. coli, C. fetus, C. lari and other Campylobacter species.

B0006 is included in P0038 rabbit fecal PCR panel and P0041 - waterborne pathogens screening panel

B0007 - Qualitative specific detection of Campylobacter jejuni only, by real time polymerase chain reaction

 

Family Campylobacteraceae includes 2 genera, Campylobacter and Arcobacter. There are 18 species and subspecies within the genus Campylobacter, 11 of which are considered pathogenic to humans, causing enteric and extra-intestinal illnesses. The major pathogens are Campylobacter jejuni and Campylobacter fetus. These pathogens are small, curved, motile, microaerophilic, gram-negative rods. They vary in width from 0.2-0.9 um and in length from 0.5-5.0 um. They exhibit rapid, darting motility in corkscrew fashion by means of a single flagellum or 2 flagella (monotrichous, amphitrichous). They also possess a lipopolysaccharide endotoxin.

Campylobacteriosis is the infectious disease caused by bacteria of the genus Campylobacter. Most people suffering from campylobacteriosis develop diarrhea, cramping, abdominal pain, and fever within 2 to 5 days after exposure to the organism. The diarrhea may be bloody and can be accompanied by nausea and vomiting. The symptoms may last for one week. Some persons infected with Campylobacter, however, may be asymptomatic. In persons with compromised immune systems, Campylobacter can occasionally spread to the bloodstream and causes a serious life-threatening infection.

People who get campylobacteriosis usually recover completely within 2 to 5 days, although sometimes recovery can take up to 10 days. Although rare, long-term consequences sometimes result from Campylobacter infection. Some people may have arthritis following campylobacteriosis; others may develop a rare disease that affects the nerves of the body beginning several weeks after the diarrheal illness. This disease, called Guillain-Barré syndrome, occurs when a person's immune system is "triggered" to attack the body's own nerves, and can lead to paralysis that lasts several weeks and usually requires intensive care. It is estimated that approximately one in 1000 reported campylobacteriosis cases leads to Guillain-Barré syndrome. As many as 40% of Guillain-Barré syndrome cases in the United States may be caused by campylobacteriosis.

Although Campylobacter bacteria isolation can be used to diagnose the bacterial infection, a long incubation period is required to obtain results. Furthermore, bacterial culture is not very sensitive nor specific, and it increases the potential risk of laboratory personnel contacting the bacteria. Subspecies identification by culture can be difficult due to new variants. Campylobacter detection by PCR is not only rapid, sensitive and specific, but can also accurately subtype the bacteria.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of campylobacter infection.
  • Help ensure that rodent colonies are free of these bacteria
  • Early prevention of spread of these bacteria among a colony
  • Minimize personnel exposure to these bacteria
  • Safety monitoring of biological products that derive from rodents

References:
Elmore, D. B., J. H. Anderson, D. W. Hird, K. D. Sanders, and N. W. Lerche (1992). Diarrhea rates and risk factors for developing chronic diarrhea in infant and juvenile rhesus monkeys. Lab. Anim. Sci. 42:356-359.
Munoz-Zanzi, C. A., M. C. Thurmond, D. W. Hird, and N. W. Lerche (1999) Effect of weaning time and associated management practices on postweaning chronic diarrhea in captive rhesus monkeys (Macaca mulatta). Lab. Anim. Sci. 49:617-621.
Sestak, K., Merritt, C.K., Borda, J., Saylor, E., Schwamberger, S.R., Cogswell, F., Didier, E.S., Didier, P.J., Plauche, G., Bohm, R.P., Aye, P.P., Alexa, P., Ward, R. and Lackner, A.A. (2003) Infectious agent and immune response characteristics of chronic enterocolitis in captive rhesus macaques. Infect Immun. 71:4079-86.
Baze, W.B. and Bernacky, B.J. (2002) Campylobacter-induced fetal death in a rhesus monkey. Vet Pathol. 39:605-7.
Kulkarni, S.P., Lever, S., Logan, J.M., Lawson, A.J., Stanley, J. and Shafi, M.S. (2002) Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods. J Clin Pathol. 55:749-753.

Specimen requirements:  1 fecal pellet, or 0.2 ml bacterial culture.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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