Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Tyzzers disease PCR test for rodents
rodent and rabbit assay data sheet

Clostridium piliforme - Tyzzer's disease

Test code: B0043 - Ultrasensitive qualitative detection of Clostridium piliforme (Tyzzer's disease) bacteria by real time PCR

B0043 is included on the Rabbit Fecal PCR Panel

Infection with Clostridium piliforme results in Tyzzer’s disease, which is characterized by necrotic lesions in the liver, digestive organs and heart. A number of animal species are susceptible to this organism, including mice, rats, rabbits, dogs, cats, primates, and horses.

The organism is an obligate gram-negative bacteria found in necrotic foci in spore forms. Transmission is mainly through the fecal-oral route.

Although Tyzzer’s is a severe disease in many animal species, infected mice often do not exhibit clinical symptoms. These mice become carriers of the disease and spread the pathogen to other mice and other animal species. Interestingly, different mouse strains differ in their susceptibility to the pathogen (Waggie et al., 1981).

Clostridium piliforme cannot be cultivated in artificial media, so diagnosis may be based on microscopic examination of tissues, serological assays or steroid challenge tests; these methods all require blood or necropsy samples. When steroid challenge assays are performed, extreme care must be taken to avoid spreading the pathogen. Moreover, microscopic examination, serology and steroid challenge all suffer from a lack of sensitivity and are labor intensive.

Detection of this pathogen by polymerase chain reaction is highly sensitive and specific. The test can be performed on fecal specimens rather than blood or tissue, resulting in less trauma and risk to animals.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Tyzzer's disease.
  • Help ensure that rodent colonies are free of Clostridium piliforme
  • Early prevention of spread of C. piliforme among a colony
  • Minimize personnel exposure to C. piliforme
  • Safety monitoring of biological products that derive from rodents

References:
Waggie, K.S., Hansen, C.T. Ganaway, J.R. and Spencer, T.S. (1981) A study of mouse strains susceptibility to Bacillus piliformis (Tyzzer's disease): the association of B-cell function and resistance. Lab. Anim. Sci. 31:139-142

Specimen requirements: 1 fecal pellet.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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