rodent and rabbit assay data sheet
Ultrasensitive qualitative detection of K virus by
real time PCR
is included on
P0029 - Mouse
Mouse K virus was first discovered by Lawrence Kilham - thus “K”
virus. It belongs to the family
virus is usually spread by the oronasal route.
When a young mouse is orally inoculated with the virus,
the virus first replicates in the intestine and then spreads to
other organs such as liver, lung, spleen and brain. Older mice
may mount an immune response to the virus and thus may limit the
spread of the virus through the body. However, athymic or nude
mice suffer significantly if infected.
In naturally infected colonies of mice, significant clinical
symptoms are usually absent and these infected mice may become
carriers of the virus (Lussier and Descôteaux,
and pass it to young mice.
Diagnosis of mice infected with K virus is sometimes done by
serology testing. However, since many mice may have had prior
exposure to this virus, a positive serology result may not
indicate current carrier status.
Molecular detection by PCR can provide rapid, highly
sensitive, highly specific identification of the presence of the
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of K virus
Help ensure that vivariums are free of K virus
Early prevention of spread of K virus among
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
Lussier, G. and Descôteaux,
J.P. (1986) Prevalence of natural virus infections in
laboratory mice and rats used in Canada. Lab. Anim. Sci.
Fecal pellet, or
0.2 ml whole blood in EDTA (purple top) or ACD
(yellow top) tube, or 0.2 ml cell culture
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
real time polymerase chain reaction
Normal range: Nondetected