Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Enterobius vermicularis

Francisella tularensis

Hantavirus

Helicobacter

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mouse adenoviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Shigella

Sialodacryoadenitis virus (SDAV)

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


K virus PCR test

rodent and rabbit assay data sheet

K virus

Test code: S0160 - Ultrasensitive qualitative detection of K virus by real time PCR

S0160 is included on P0029 - Mouse Essentials Panel

Mouse K virus was first discovered by Lawrence Kilham - thus “K” virus. It belongs to the family Pavovaviridae. The virus is usually spread by the oronasal route.  When a young mouse is orally inoculated with the virus, the virus first replicates in the intestine and then spreads to other organs such as liver, lung, spleen and brain. Older mice may mount an immune response to the virus and thus may limit the spread of the virus through the body. However, athymic or nude mice suffer significantly if infected.

In naturally infected colonies of mice, significant clinical symptoms are usually absent and these infected mice may become carriers of the virus (Lussier and Descôteaux, 1986) and pass it to young mice.

Diagnosis of mice infected with K virus is sometimes done by serology testing. However, since many mice may have had prior exposure to this virus, a positive serology result may not indicate current carrier status.  Molecular detection by PCR can provide rapid, highly sensitive, highly specific identification of the presence of the virus.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of K virus
  • Help ensure that vivariums are free of K virus
  • Early prevention of spread of K virus among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

References:
Lussier, G. and Descôteaux,  J.P. (1986) Prevalence of natural virus infections in laboratory mice and rats used in Canada. Lab. Anim. Sci. 36:145-148.

Specimen requirements: Fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cell culture

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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