rodent and rabbit assay data sheet
Lymphocytic choriomeningitis virus (LCMV)
S0142 - Ultrasensitive qualitative detection of lymphocytic
choriomeningitis virus (LCMV) by reverse transcription coupled
real time PCR
included on P0029 - Mouse
Lymphocytic choriomeningitis virus (LCMV) is an
enveloped negative-sense single-stranded RNA virus belonging to
the family Arenaviridae. LCMV is naturally spread by the common
house mouse, Mus musculus. Infected mice can become
chronically infected and carry a low level of virus in their
blood. These carriers can spread the virus to other animals
by shedding virus in their urine or from mothers to their
offspring. About five per cent of mice, hamsters and rodents are
thought to carry the disease. Pet rodents can contract the virus
after exposure to infected mice.
The virus is
resistant to drying and can survive for prolonged periods in the
environment. Humans can become infected by inhaling aerosolized
particles of dried urine, feces or saliva from infected rodents;
by ingesting virus-contaminated food; by contact of mucus
membranes with infected body fluids; or by direct exposure of
cuts or other open wounds to contaminated materials. Infected
humans can develop aseptic meningitis, encephalitis or
are rarely infected by contacting pet rodents, approximately 5%
of the US population are serologically reactive to LCMV.
Seroprevalence tends to be more common among lower
socio-economic groups, probably due to more frequent and direct
contacts with mice. The virus normally has little effect on
healthy people but can be deadly for people whose immune system
has been weakened (Fischer, 2008). Person-to-person transmission
does not usually occur, with the exception of vertical
transmission from infected mother to fetus.
detection of LCMV in rodents is not very sensitive because viral
titer in the blood of infected animals may be very low. Urine
and fecal samples are convenient samples for screening for
infected mice, but serology testing cannot be applied to these
sample types. In addition, serology testing is difficult to use
for environmental surveys for the virus. Molecular detection of
this virus by PCR is a rapid, sensitive and specific methodology
that can be applied to a wide variety of sample types.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of LCMV
Help ensure that vivariums are free of LCMV
Early prevention of spread of this virus among a
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
Fischer, S.A. (2008) Emerging viruses in transplantation: there
is more to infection after transplant than CMV and EBV.
Transplantation, 86: 1327-1329.
Fecal pellet, or 0.2 ml urine, or 0.2 ml whole blood in EDTA
(purple top) or ACD (yellow top) tube,
or 0.2 ml cell culture.
For specimen types other than those listed here, please call to
confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
for more information.
2 business days
reverse transcription coupled real time polymerase chain