Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Lymphocytic choriomeningitis virus (LCMV) PCR test for rodents

rodent and rabbit assay data sheet

 

Lymphocytic choriomeningitis virus (LCMV)

Test code: S0142 - Ultrasensitive qualitative detection of lymphocytic choriomeningitis virus (LCMV) by reverse transcription coupled real time PCR

S0142 is included on P0029 - Mouse Essentials Panel

Lymphocytic choriomeningitis virus (LCMV) is an enveloped negative-sense single-stranded RNA virus belonging to the family Arenaviridae. LCMV is naturally spread by the common house mouse, Mus musculus. Infected mice can become chronically infected and carry a low level of virus in their blood. These carriers can spread the virus to other animals by shedding virus in their urine or from mothers to their offspring. About five per cent of mice, hamsters and rodents are thought to carry the disease. Pet rodents can contract the virus after exposure to infected mice.

The virus is resistant to drying and can survive for prolonged periods in the environment. Humans can become infected by inhaling aerosolized particles of dried urine, feces or saliva from infected rodents; by ingesting virus-contaminated food; by contact of mucus membranes with infected body fluids; or by direct exposure of cuts or other open wounds to contaminated materials. Infected humans can develop aseptic meningitis, encephalitis or meningoencephalitis.

Although humans are rarely infected by contacting pet rodents, approximately 5% of the US population are serologically reactive to LCMV. Seroprevalence tends to be more common among lower socio-economic groups, probably due to more frequent and direct contacts with mice. The virus normally has little effect on healthy people but can be deadly for people whose immune system has been weakened (Fischer, 2008). Person-to-person transmission does not usually occur, with the exception of vertical transmission from infected mother to fetus.

Serological detection of LCMV in rodents is not very sensitive because viral titer in the blood of infected animals may be very low. Urine and fecal samples are convenient samples for screening for infected mice, but serology testing cannot be applied to these sample types. In addition, serology testing is difficult to use for environmental surveys for the virus. Molecular detection of this virus by PCR is a rapid, sensitive and specific methodology that can be applied to a wide variety of sample types.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of LCMV
  • Help ensure that vivariums are free of LCMV
  • Early prevention of spread of this virus among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

References:
Fischer, S.A. (2008) Emerging viruses in transplantation: there is more to infection after transplant than CMV and EBV. Transplantation, 86: 1327-1329.

Specimen requirements: Fecal pellet, or 0.2 ml urine, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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