Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mouse adenovirus PCR test

rodent and rabbit assay data sheet

Mouse adenovirus 1 and 2 (MAD-1 and MAD-2)

Test codes:
S0146
- Ultrasensitive qualitative detection of mouse adenovirus 1 (MAD-1) by real time PCR
S0147
- Ultrasensitive qualitative detection of mouse adenovirus 2 (MAD-2) by real time PCR

S0146 and S0147 are included on P0029 - Mouse Essentials Panel

Mouse adenoviruses are DNA viruses; there are two strains, group 1 (MAD-1) and group 2 (MAD-2). These viruses infect both mice and rats.

Infection with these viruses does not cause clinical disease in adult rodents, and there are no pathologic lesions associated with infections of MAD-1 in adult mice. However, MAD-1 infection can produce a lethal disease in newborn or suckling mice characterized by infectious virus and viral lesions in multiple organs. Viral inclusions in intestinal mucosa are associated with MAD-2 infections.

Infection of mice or rats with adenoviruses can alter their normal immune response and thereby skew experimental data. For example, infection with MAD-1 can produce extensive persistent lesions in the kidneys of adult mice and render them more susceptible to experimental Escherichia coli-induced pyelonephritis. Mouse adenovirus infection has also been shown to accelerate experimental scrapie infection in mice. Although mouse adenoviral infection is usually subclinical in immunocompetent mice, wasting may result in nude mice.

Serological detection may not be useful because many mice and rats may have prior exposure to these viruses. Hemagglutination methodology is also not suitable for mouse adenovirus detection because these viruses simply will not hemagglutinate. Molecular detection of adenoviruses is rapid, specific and sensitive to detect the presence of the viruses.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of mouse adenoviruses
  • Help ensure that vivariums are free of mouse adenoviruses
  • Early prevention of spread of mouse adenoviruses among a vivarium
  • Minimize personnel exposure to these viruses
  • Safety monitoring of biological products that derive from mice

References:
Implications of infectious agents on results of animal experiments. Report of the Working Group on Hygiene of the Gesellschaft für Versuchstierkunde--Society for Laboratory Animal Science (GV-SOLAS). Lab. Animal. 1999; 33 Suppl 1:S39-87.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube; or 0.2 ml tissue, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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