Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mouse cytomegalovirus PCR test

rodent and rabbit assay data sheet

Mouse cytomegalovirus type 1 and type 2 (MCMV-1 and MCMV-2)

Test codes:
S0163
- Ultrasensitive qualitative detection of murine cytomegalovirus type 1 (MCMV1) by real time PCR.
S0164 - Ultrasensitive qualitative detection of murine cytomegalovirus type 2 (MCMV2) by real time PCR.
S0170 - Ultrasensitive qualitative detection but not differentiation of murine cytomegalovirus type 1 (MCMV1) and type 2 (MCMV2) by real time PCR.

S0163 and S0164 are included on P0029 - Mouse Essentials Panel

Mouse cytomegalovirus (MCMV) is a member of the beta herpes virus family and is a large, double stranded DNA virus with a genome size of approx 230 kb. Similarly to rat CMV, two strains of MCMV have been described: MCMV1, and more recently MCMV2 (Teterinal et al., 2009).

MCMV has broad tissue tropism, and can infect the host’s epithelial cells such as salivary gland tissue, as well as macrophages and lymphoid cells. In natural infections, no clinical symptoms may be detected. The submandibular salivary gland is the primary site of lesions; other salivary glands are rarely involved. A substantial percentage of infected mice may eventually develop latent infections, and the virus may be transmitted to other mice through biting.

Wild mice remain the reservoir for the virus. Approximately 90% of wild mice are infected with MCMV. Thus, vivariums should be equipped with anti-rodent barriers to prevent the mouse colonies from coming into contact with wild mice.

Both serological and molecular detection methods have been used to diagnose MCMV infection. However, molecular detection by PCR is especially useful not only because it is highly sensitive and specific but also because it can identify mice that are latent carriers of the virus.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of MCMVs
  • Help ensure that vivariums are free of MCMVs
  • Early prevention of spread of murine cytomegaloviruses among a vivarium
  • Minimize personnel exposure to these viruses
  • Safety monitoring of biological products that derive from mice

Specimen requirements: Fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cell culture

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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