rodent and rabbit assay data sheet
Mouse hepatitis virus (MHV)
S0034 - Ultrasensitive qualitative detection of mouse hepatitis
virus by reverse transcription coupled real time PCR
is included on P0029 - Mouse
P0032 Mouse Minipanel
hepatitis virus (MHV) is single-stranded RNA virus of the family
Coronaviridae. MHV is not a single virus but consists of a group
of serologically and genetically related but distinct strains.
About 25 strains have been reported. Strains are characterized
by cross-neutralization tests with known strains, but these
serologic relationships do not correlate with pathogenicity. The
best studied strains are the "prototype strains" MHV-1, MHV-2,
MHV-3, JHM, A59 and S, of which MHV-3 is regarded as the most
virulent. Field isolates often differ more or less from these.
MHV mutates rapidly and there may be a large possible number of
"strains". Strains differ in tropisms, tendency to disseminate,
and virulence. Although infection generally causes no
significant clinical symptoms, especially in adult mice, it can
dramatically affect the immune system of the animal,
complicating interpretation of many experimental results.
extremely contagious and is transmitted primarily via aerosol,
direct contact and fomites. Mice with active infection shed
virus in feces and in oral and nasal secretions.
rodents infected with the virus are usually asymptomatic
carriers. Specific clinical signs depend on the strain and are
most evident in infant mice. Typical symptoms may include
diarrhea, poor growth, and death. Immunodeficient mice, such as
athymic nude mice, can develop a wasting disease as a result of
the infection that eventually leads to death.
can be based on ELISA or other serological techniques, but these
are inadequate in acute outbreaks because infected mice may not
develop ELISA-detectable antibodies for up to 10 or more days
following initial exposure. Rapid spread of the virus through a
colony can thus occur if the infection is unnoticed. To protect
against such cases, direct detection by highly sensitive and
specific reverse transcription coupled PCR is useful.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of MHV
Help ensure that vivariums are free of mouse hepatitis virus
Early prevention of spread of mouse hepatitis virus
among a vivarium
Minimize human exposure to this virus
Safety monitoring of biological products that derive
Fecal pellet, or oral swab,
or 0.2 ml cell culture.
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
reverse transcription coupled real time polymerase chain