Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mouse hepatitis PCR test

rodent and rabbit assay data sheet

Mouse hepatitis virus (MHV)

Test code: S0034 - Ultrasensitive qualitative detection of mouse hepatitis virus by reverse transcription coupled real time PCR

 S0034 is included on P0029 - Mouse Essentials Panel and on P0032 Mouse Minipanel


Mouse hepatitis virus (MHV) is single-stranded RNA virus of the family Coronaviridae. MHV is not a single virus but consists of a group of serologically and genetically related but distinct strains. About 25 strains have been reported. Strains are characterized by cross-neutralization tests with known strains, but these serologic relationships do not correlate with pathogenicity. The best studied strains are the "prototype strains" MHV-1, MHV-2, MHV-3, JHM, A59 and S, of which MHV-3 is regarded as the most virulent. Field isolates often differ more or less from these. MHV mutates rapidly and there may be a large possible number of "strains". Strains differ in tropisms, tendency to disseminate, and virulence. Although infection generally causes no significant clinical symptoms, especially in adult mice, it can dramatically affect the immune system of the animal, complicating interpretation of many experimental results.

MHV is extremely contagious and is transmitted primarily via aerosol, direct contact and fomites. Mice with active infection shed virus in feces and in oral and nasal secretions.

Adult rodents infected with the virus are usually asymptomatic carriers. Specific clinical signs depend on the strain and are most evident in infant mice. Typical symptoms may include diarrhea, poor growth, and death. Immunodeficient mice, such as athymic nude mice, can develop a wasting disease as a result of the infection that eventually leads to death.

Diagnosis can be based on ELISA or other serological techniques, but these are inadequate in acute outbreaks because infected mice may not develop ELISA-detectable antibodies for up to 10 or more days following initial exposure. Rapid spread of the virus through a colony can thus occur if the infection is unnoticed. To protect against such cases, direct detection by highly sensitive and specific reverse transcription coupled PCR is useful.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of MHV
  • Help ensure that vivariums are free of mouse hepatitis virus
  • Early prevention of spread of mouse hepatitis virus among a vivarium
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from mice

Specimen requirements: Fecal pellet, or oral swab, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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