Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mouse norovirus PCR test

rodent and rabbit assay data sheet

Mouse norovirus (MNV)

Test code: S0138 - Ultrasensitive qualitative detection of mouse norovirus by reverse transcription coupled real time PCR

S0138 is included on P0023 - Mouse Fecal Panel, P0029 - Mouse Essentials Panel and P0032 Mouse Minipanel


Noroviruses are a group of genetically related and antigenically diverse viruses that can infect both humans and animals. They are caliciviruses and carry positive single-stranded RNA. These viruses are resistant to organic solvents and mild detergents and hence can survive in the environment for some time. Transmission occurs by ingestion of material contaminated by infected feces. These viruses infect and replicate in dendritic cells of the gut and in macrophage cells.

Mouse norovirus was first identified in 2003. Immunocompetent mice infected with the virus can develop transient infection with a short duration of fecal shedding of the virus. Mice that are immunocompromised, including some knockout strains, can develop lethal infection. Sharing of mice between animal facilities can significantly increase the risk of spreading the infection.

Serologic assays can be used to detect exposure of mice to the virus, but it usually takes a long time for detectable serological changes to occur.  However, even recently-infected mice will shed the virus in feces, allowing viral RNA to be readily detected by reverse transcription coupled real time PCR (Kelmenson et al., 2009).  PCR detection is highly sensitive, specific and rapid; this technique provides a valuable tool for screening mice being moved between facilities.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of norovirus
  • Help ensure that vivariums are free of norovirus
  • Early prevention of spread of norovirus among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

References:
Kelmenson, J.A., Pomerleau, D.P., Griffey, S., Zhang, W., Karolak, M.J. and Fahey, J.R. (2009) Kinetics of transmission, infectivity, and genome stability of two novel mouse norovirus isolates in breeding mice. Comp. Med. 59:27-36

Specimen requirements: 1 fecal pellet, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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