rodent and rabbit assay data sheet
Mouse rotavirus (EDIM)
S0097 - Ultrasensitive qualitative detection of mouse rotavirus
(EDIM) by reverse transcription coupled real time PCR.
is included on P0029 - Mouse
P0032 Mouse Minipanel
young laboratory mice is often caused by mouse rotavirus, also
called epizootic diarrhea of infant mice (EDIM). This RNA virus
is highly contagious and is transmitted via contaminated
bedding, airborne dust, and through contact with infected mice.
The virus is shed in the feces for about 10 days post-infection.
Animals are most susceptible between 0 and 14 days of age.
mice, usually under 14 days of age, will develop symptoms such
as watery and mustard-colored stools, lethargy, and distended
abdomens. Rectal impaction may occur at 12 to 16 days of age. If
the impacted fecal material is not removed spontaneously or
deliberately, the animals will die.
can alter host physiology in multiple ways and can significantly
complicate the interpretation of research findings. Examples of
such alterations include increased susceptibility to the
pathologic effects of copathogens, altered results of dietary
and nutritional studies and alterations in gastrointestinal
based on serology (eg ELISA) is inadequate in a number of cases,
such as in acute outbreaks when affected mice may not develop
ELISA-detectable antibodies for several days after initial
exposure, resulting in rapid, undetected spread of the virus
through a colony. Direct detection of viral RNA by reverse
transcription PCR is useful in such cases.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of mouse rotavirus
Help ensure that vivariums are free of mouse rotavirus
Early prevention of spread of mouse rotavirus among a
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
or 0.2 ml cell culture.
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
reverse transcription coupled real time polymerase chain