Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mousepox ectromelia PCR test for rodents

rodent and rabbit assay data sheet

Mousepox virus (aka ectromelia, EV, ECTV, ECTR or ECTRO)

Test code: S0144 - Ultrasensitive qualitative detection of mousepox virus by real time PCR

S0144 is included on P0029 - Mouse Essentials Panel

 Mousepox virus, also known as ectromelia virus, is a double-stranded DNA virus belonging to the poxvirus family. The virus replicates in the cell cytoplasm and it encodes its own replication and transcription machinery. Members of the poxvirus family include variola virus, one of the most virulent human pathogens that caused smallpox, and vaccinia virus, the smallpox vaccine.

The mousepox virus is a highly contagious pathogen of mice and rats that causes mousepox, a severe disease with high mortality rate. Outbreaks in laboratory mouse colonies have often been reported and have caused serious disruptions in biomedical research.

Close contact among mice and rats can spread the virus easily. Natural infections can occur via the fecal-oral route and urine contamination. Newly infected mice may develop pustules in approximately 10 days which often resemble bite marks. Resulting necrosis may lead to the loss of digits and limbs and destruction of liver and lymphoid tissue. Mortality is very high and can reach 100%. Many infected animals, however, may develop latent infections with no clinical symptoms which can be reactivated by stressors such as irradiation and transport. Upon necropsy an enlarged spleen and intestinal hemorrhaging are often noted.

Diagnosis of the disease has often been based on its distinctive lesions (Roberts and Carter, 1976) but this approach is not reliable because some latently-infected mice and rats may not have symptoms. Serological detection may indicate prior exposure but cannot confirm the current presence of virus in those latently-infected rodents. Molecular detection is a rapid, sensitive and specific method to detect and confirm the presence of mousepox virus.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of mousepox
  • Help ensure that vivariums are free of mousepox virus
  • Early prevention of spread of this virus among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from rodents

References:
Roberts, W.A. and Carter, G.A.(1976) Essentials of veterinary virology, p. 171. Michigan State University Press, East Lansing, Mich.

Specimen requirements: Fecal pellet, or 0.2 ml fresh, frozen or fixed tissue, or skin scrapings, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml urine, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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