Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Mycoplasma PCR tests for rodents

rodent and rabbit assay data sheet

Mycoplasma

Test codes:

B0002 - Ultrasensitive qualitative detection of Mycoplasma pneumoniae by real time polymerase chain reaction.

B0009 - Ultrasensitive Mycoplasma screen by real time PCR.  This screen detects but does not differentiate M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. orale, M. pirum, M. salivarium, M. agassizii, M. cynos and others. This screen does not detect M. pneumoniae or M. pulmonis.

B0041 - Ultrasensitive qualitative detection of Mycoplasma pulmonis by real time polymerase chain reaction

 B0041 is included on P0029 - Mouse Essentials Panel

Murine respiratory mycoplasmosis in mice and rats is caused by Mycoplasma pulmonis. In addition to exhibiting high morbidity and reduced birth rate, mice and rats infected with M. pulmonis often develop imperceptible infections. These can cause problems in research studies using these animals, as physiological mechanisms and the immune system may be affected. Experimental results obtained with infected animals can be misleading.

Diagnosis of M. pulmonis infection can be done using serological methods such as microbial isolation or enzyme linked immunosorbent assay (ELISA) (Cassell et al., 1981). However, in vitro isolation is time-consuming, and serological methods often give incorrect results due to cross-reactivity between different species of rodent mycoplasmas (Cassell et al., 1981; Davis et al., 1987; Davidson et al., 1994). Serological assays also lack sensitivity because a low level of antibody -- early in an infection or in immunodeficient animals -- may not be detected.

Detection of this pathogen by polymerase chain reaction is especially important because it is very sensitive and specific. The result will not be compromised if the infection is at an early stage or the animal is immunodeficient.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of M. pulmonis infection.
  • Help ensure that rodent colonies are free of M. pulmonis
  • Early prevention of spread of M. pulmonis among a colony
  • Minimize personnel exposure to M. pulmonis
  • Safety monitoring of biological products that derive from rodents

References:
Cassell, G.H., Lindsey, J.R., Davis, J.K., Davidson, M.K., Brown, M.B. and Mayo, J.G. (1981) Detection of natural Mycoplasma pulmonis infection in rats and mice by an enzyme linked immunosorbent assay (ELISA). Lab Anim Sci 31: 676–682.
Davis, J.K., Cassell, G.H., Gambill, G., Cox, N., Watson, H. and Davidson, M. (1987) Diagnosis of murine mycoplasmal infections by enzyme-linked immunosorbent assay (ELISA). Isr J Med Sci 23: 717–722.
Davidson, M.K, Davis, J.K., Gambill, G.P., Cassell, G.H. and Lindsey, J.P. (1994) Mycoplasmas of laboratory rodentsMycoplasmas in Animals: Laboratory Diagnosis, Iowa State University Press, Ames p. 97–133.

Specimen requirements:

B0009 - 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or vaginal swab, or 0.2 ml bacterial culture or cell culture.

B0002 or B0041 - Nasal swab, or 0.2 ml bacterial culture or cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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