Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Pasteurella multocida PCR test for rodents
rodent and rabbit assay data sheet

Pasteurella multocida

Test code: B0045 - Ultrasensitive qualitative detection of Pasteurella multocida by real time PCR

 

Pasteurella bacteria are small, nonmotile, gram-negative, bipolar-staining bacilli that normally inhabit the nasal, gingival and tonsillar regions of most domestic cats, many dogs and many other animal species.

Pasteurella secretes an endotoxin that changes the properties of the pulmonary surfactant. This alters pulmonary mechanics and gas exchange, often resulting in a pneumonia which is slow to resolve. Abscesses or pleuritis may also result from Pasteurella infection.

Pasteurella multocida is the most common respiratory pathogen in the domestic rabbit. Infection with the bacteria can result in rhinitis, conjunctivitis, pneumonia, abscesses, genital tract infections, and septicemia. The bacteria can be transmitted via aerosol or contact with an infected animal, either directly or through fomites. P. multocida initially colonizes the pharynx, then moves to the nasal cavity and surrounding tissue, with the potential to spread to the rest of the body. Colonization of the nasal cavity may take two weeks to occur, at which time clinical signs may or may not appear. Infected rabbits may become carriers without exhibiting clinical signs.

Traditionally, diagnosis of Pasteurella was based on clinical findings, culture and/ or serological testing. Although culture identification methods are definitive, they are time consuming and costly. False-negative culture results are frequently observed due to the fact that P. multocida dies easily during transport to the laboratory or is overgrown by other bacteria (nasal flora and contaminants) in the culture.

Serology can be used for cases when infection is suspected in organs for which cultures are not attainable, or when culturing has yielded no results. However, a seropositive titer to P. multocida merely indicates past exposure to the organism. Because many rabbits and other animals have been exposed to this organism, a diagnosis of pasteurellosis cannot be made based on serologic results alone. Molecular detection by PCR, however, offers a highly sensitive, accurate and timely method for detecting Pasteurella and confirming current infection (Miflin and Blackall, 2001).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Pasteurella
  • Help ensure that rabbit facilities are free of Pasteurella
  • Early prevention of spread of Pasteurella among a facility
  • Minimize personnel exposure to Pasteurella
  • Safety monitoring of biological products that derive from rabbits and other animals

References:
Miflin, J. K. and Blackall, P. J. (2001) Development of a 23 S rRNA-based PCR assay for the identification of Pasteurella multocida. Lett. Appl. Microbiol. 33: 216–221.

Specimen requirements: Nasal swab or 0.2 ml whole blood in EDTA.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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