Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Reovirus PCR screen for rodents

rodent and rabbit assay data sheet

Reovirus screen

Test code S0120  - Ultrasensitive qualitative screen for mammalian reoviruses by reverse transcription coupled real time PCR.  This assay detects a wide range of mammalian reoviruses but does not detect avian reovirus strains.

S0120 is included on P0029 - Mouse Essentials Panel


Reoviruses are comprised of 10 to 12 double-stranded RNA genomic segments that can reassort both in nature and in laboratory settings. The most common mammalian isolates are type 1 (Lang), type 2 (Jones), and type 3 (Dearing).

Reoviruses have a high endemic infection rate in many mammals, such as primates, cattle, cats, dogs, rodents and swine. These viruses are common in raw water sources and are often found along with other animal viruses. In humans, the viruses cause only asymptomatic or mild respiratory infections. However, research suggests that reoviruses may be associated with potentially more severe illnesses. Reoviruses have been linked to neonatal hepatitis, extrahepatic biliary atresia, meningitis and myocarditis. Also, immunocompromised, young and elderly individuals may become susceptible to severe bacterial respiratory disease due to an initial reovirus infection.

Due to their widespread occurrence and the ability of these viruses to survive a long period of time in the environment, contamination of water sources has been frequently reported. Animals are especially prone to infection by these viruses. Xenotransplantation of animal organs is severely endangered by potential contamination with these viruses.

Diagnosis of reovirus infection by nonmolecular means is very difficult and is usually based on virus isolation on cell cultures and electron microscopy. These methods are not very sensitive (Muscillo et al., 2001) and are likely underestimate the presence of these viruses in animals and humans. Molecular detection by PCR is the most sensitive, rapid and specific method for identifying reoviruses.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that rodent colonies are free of reoviruses
  • Early prevention of spread of reoviruses among a colony
  • Minimize human exposure to reoviruses
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Muscillo M., La Rosa G., Marianelli C., Zaniratti S., Capobianchi M.R., Cantiani L. and Carducci A. (2001) A new RT-PCR method for the identification of reoviruses in seawater samples, Water Res. 35:548–556.

Specimen requirements: Tracheal swab, or nasal swab, or 1 fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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