Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Sendai PCR test

rodent and rabbit assay data sheet

Sendai virus (SEND)

Test code: S0099 - Ultrasensitive qualitative detection of Sendai virus by reverse transcription coupled real time PCR

 S0099 is included on P0029 - Mouse Essentials Panel

Sendai virus (SEND) is an RNA paramyxovirus of the parainfluenza type 1 group. Mice infected with this virus develop labored breathing and decreased fecundity. In DBA/2 and immune deficient mice, the infection is almost always fatal. This virus immunosuppresses affected mice and predisposes them to secondary bacterial infections.

Generally, no clinical signs are observed in mice in endemically infected colonies. Thus many mice may be carriers of the virus. In clinically apparent infections, signs are variable but may include chattering, mild respiratory distress to labored breathing, and decreased fecundity in adults. Deaths (possibly whole litters) in neonates and suckling mice can occur due to the weak immune defense at this age. In young adult mice, poor growth is common.

Direct contact is the primary means of viral spread. The virus is not environmentally stable, but can be transmitted by fomites because of the quantities of virus excreted from infected mice.

Serology testing for these viruses has been used extensively to test for the immune status of mice. However, this testing approach is often inadequate to control the spread of the virus, largely because it takes a long time to generate a detectable serological response when the mice are exposed to this virus. Secondly, the sensitivity of serological testing is often not comparable to molecular detection by PCR. Thirdly, serological testing cannot be applied to immunosuppressed mice, such as nude mice. Fourthly, swabs can be conveniently used by PCR to detect the presence of Sendai virus.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Sendai virus
  • Help ensure that vivariums are free of Sendai virus
  • Early prevention of spread of Sendai virus among a vivarium
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

Specimen requirements: Nasopharyngeal swab, or 0.2 ml fresh or frozen tracheal or lung tissue, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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