Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Theilers disease PCR test

rodent and rabbit assay data sheet

Theiler's murine encephalomyelitis virus (TMEV)

Test code: S0102 - Ultrasensitive qualitative detection of TMEV by reverse transcription coupled real time PCR

 S0102 is included on P0029 - Mouse Essentials Panel and on P0032 Mouse Minipanel

Theiler’s murine encephalomyelitis virus (TMEV) is a single-stranded RNA picornavirus that persistently infects the murine central nervous system. The virus has recently been reclassified into the cardiovirus group. Mice are the natural host of this virus. In the wild it produces a gastrointestinal infection that may be complicated by concomitant infection of the nervous system.

There are two main groups of TMEV. One includes the GDVII and FA viruses, which produce an acute fulminant encephalomyelitis, which is fatal in about a week. The other, called Theiler’s original group, includes the DA, BeAn, and WW viruses. These produce a chronic persistent central nervous system infection, accompanied by inflammatory demyelinating lesions of the spinal cord with features very similar to those of demyelinating plaques seen in human multiple sclerosis. The demyelination induced by TMEV infection is mediated by the immune system rather than as a consequence of viral infection of oligodendrocytes, the myelin forming cells.

A classic study (Theiler, 1937) showed that mice infected with the virus may not show prominent symptoms and become carriers of the virus. The virus can be transmitted easily through soiled bedding.  Infected immunocompetent mice may shed TMEV in the feces for as long as 53 days; the duration of shedding by immunodeficient mice has not been reported but is likely longer (Clifford and Watson, 2008).

Serological detection of the virus is inadequate, especially in nude or immunocompromised mice. Furthermore, neonatal mice infected with the virus may not be readily identified due to residual maternal antibodies. Molecular detection by PCR can overcome these problems and is highly sensitive and specific for TMEV.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of TMEV
  • Help ensure that rodent facilities are free of TMEV
  • Early prevention of spread of this virus among a rodent facility
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products that derive from mice

References:
Theiler, M. (1937) Spontaneous encephalomyelitis of mice, a new virus disease, J. Exp. Med. 65: 705–719.

Clifford, C.B. and Watson, J. (2008) Old Enemies, Still with Us After All These Years, ILAR Journal Vol. 49, No. 3: 291-301.

Specimen requirements: Fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml CSF, or 0.2 ml fresh or frozen tissue, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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