rodent and rabbit assay data sheet
Theiler's murine encephalomyelitis virus (TMEV)
Ultrasensitive qualitative detection of TMEV by reverse
transcription coupled real time PCR
murine encephalomyelitis virus (TMEV) is a single-stranded RNA
picornavirus that persistently infects the murine central
nervous system. The virus has recently been reclassified into
the cardiovirus group. Mice are the natural host of this virus.
In the wild it produces a gastrointestinal infection that may be
complicated by concomitant infection of the nervous system.
is included on P0029 - Mouse
P0032 Mouse Minipanel
There are two
main groups of TMEV. One includes the GDVII and FA viruses,
which produce an acute fulminant encephalomyelitis, which is
fatal in about a week. The other, called Theiler’s original
group, includes the DA, BeAn, and WW viruses. These produce a
chronic persistent central nervous system infection, accompanied
by inflammatory demyelinating lesions of the spinal cord with
features very similar to those of demyelinating plaques seen in
human multiple sclerosis. The demyelination induced by TMEV
infection is mediated by the immune system rather than as a
consequence of viral infection of oligodendrocytes, the myelin
A classic study
(Theiler, 1937) showed that mice infected with the virus may not
show prominent symptoms and become carriers of the virus. The
virus can be transmitted easily through soiled bedding.
Infected immunocompetent mice may shed TMEV in the feces for as
long as 53 days; the duration of shedding by immunodeficient
mice has not been reported but is likely longer (Clifford and
detection of the virus is inadequate, especially in nude or
immunocompromised mice. Furthermore, neonatal mice infected with
the virus may not be readily identified due to residual maternal
antibodies. Molecular detection by PCR can overcome these
problems and is highly sensitive and specific for TMEV.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of TMEV
Help ensure that rodent facilities are free of TMEV
Early prevention of spread of this virus among a
Minimize personnel exposure to this virus
Safety monitoring of biological products that derive
Theiler, M. (1937) Spontaneous encephalomyelitis of mice, a new
virus disease, J. Exp. Med. 65: 705–719.
Clifford, C.B. and Watson, J. (2008) Old Enemies, Still
with Us After All These Years, ILAR Journal Vol. 49, No. 3:
Fecal pellet, or
0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml CSF, or 0.2 ml fresh or frozen tissue,
or 0.2 ml cell culture.
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
reverse transcription coupled real time polymerase chain
Normal range: Nondetected