Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Tickborne encephalitis virus PCR test

rodent and rabbit assay data sheet

Tickborne encephalitis virus (TBEV)

Test code: S0184 - Ultrasensitive qualitative detection of tickborne encephalitis virus by reverse transcription coupled real time PCR

Tick-borne encephalitis virus (TBEV) belongs to the genus Flavivirus in the family Flaviviridae. Infections can result in acute central nervous system disease in humans and animals. Three subtypes of TBEV have been reported: far eastern (FE), Siberian (Sib) and European (Eu) subtypes, which are distributed over a wide area of Europe and Asia. The tick vector of the FE and Sib subtypes is Ixodes persulcatus and that of the Eu subtype is Ixodes ricinus.

Besides transmission through tick bites, the virus can also be passed on by ingestion of unpasteurized dairy products such as milk and cheese from infected goats, sheep, or cows. Transmission through laboratory exposure, breastfeeding, blood transfusion, and contact with slaughtered viremic animals has also been reported, but these modes of transmission are rare.

Human infections can develop a biphasic course involving acute febrile illness, then a period of apparent recovery, followed by neurological symptoms including headache, meningitis, meningoencephalitis and meningoencephalomyelitis. Death can occur within 5 to 7 days from the onset of neurological signs. However, many viral infections can cause similar symptoms, so laboratory confirmation is needed.

Culture detection of the virus is not efficient and takes a long time. Antigen detection has low sensitivity. Molecular detection by polymerase chain reaction (PCR) is highly specific and sensitive, and can be a good alternative to traditional methods (Rudenko et al., 2004).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of TBEV
  • Identify rodents carrying TBEV
  • Help ensure that rodent populations are free of TBEV
  • Early prevention of spread of this virus in a geographic area
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from rodents

References:
Rudenko, N., Golovchenko, M., Cihlárova, V. and Grubhoffer, L. (2004) Tick-borne encephalitis virus-specific RT-PCR--a rapid test for detection of the pathogen without viral RNA purification. Acta Virol. 48:167-171.

Specimen requirements: Whole blood in EDTA (purple top) or ACD (yellow top) tube, or cerebrospinal fluid, or fresh or frozen tissue, or culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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