Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

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Zoologix also performs rodent PCR tests for...

Aspiculuris tetraptera

Bordetella

Campylobacter

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit fibroma virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi

Tularemia

Tyzzer's disease

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Yersinia pseudotuberculosis PCR test

rodent and rabbit assay data sheet

Yersinia pseudotuberculosis

Test code: B0062 - Ultrasensitive qualitative detection of Yersinia pseudotuberculosis by real time PCR.

Yersinia pseudotuberculosis is a gram-negative bacterium which primarily causes disease in animals, although humans may also be infected through food contamination. Infection with this pathogen can cause tuberculosis-like symptoms, including localized tissue necrosis and granulomas in the spleen, liver, and lymph node.

The bacteria occur widely in various host species, including dogs, cats, horses, cattle, rabbits, deer, rodents, and birds. Documented cases of occupational exposure to Y. pseudotuberculosis include butchers working in abattoirs slaughtering swine. Outbreaks of Y. pseudotuberculosis in animal facilities have occasionally been reported, including in primate facilities (Bronson et al., 1972; Iwata et al., 2008).

Successfully culturing Y. pseudotuberculosis is very difficult, and serologic tests in many cases require serial testing on multiple serum samples taken at several time points. Molecular detection by PCR offers a rapid, sensitive and specific method for detecting Y. pseudotuberculosis.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Yersinia pseudotuberculosis
  • Help ensure that animal facilities are free of Yersinia pseudotuberculosis
  • Early prevention of spread of Yersinia pseudotuberculosis among a facility
  • Minimize human exposure to Yersinia pseudotuberculosis
  • Safety monitoring of biological products that derive from susceptible animals

References:
Bronson, R.T., May, B.D. and Ruebner, B.H. (1972) An Outbreak of Infection by Yersinia pseudotuberculosis in Nonhuman Primates. Am. J. Pathol. 69: 289–308.
Iwata, T., Une, Y., Okatani, A.T., Kato, Y., Nakadai, A., Lee, K., Watanabe, M., Taniguchi, T., Elhelaly, A.E., Hirota, Y. and Hayashidani, H. (2008) Virulence characteristics of Yersinia pseudotuberculosis isolated from breeding monkeys in Japan. Vet Microbiol. 129:404-409.

Specimen requirements:  Fecal pellet, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative polymerase chain reaction

Normal range: Nondetected

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