Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

* * *

Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Canine distemper PCR test
wildlife and zoo assay data sheet

Canine distemper virus (CDV)

Test code:
S0092 - Ultrasensitive qualitative detection of canine distemper virus (CDV) by reverse transcription coupled real time polymerase chain reaction

                                           

Canine distemper (CD) is a highly contagious disease in young dogs, particularly those 3 to 6 months of age. It has a high morbidity and mortality rate. The disease can be spread by aerosol infection (Appel and Gillespie, 1972) and is characterized by a diphasic fever curve and acute rhinitis, and later by bronchitis, catarrhal pneumonia, severe gastroenteritis, and nervous signs.

The causative agent of the disease is a virus belonging to the genus Morbillivirus of family Paramyxoviridae. Since the canine distemper virus (CDV) can survive for a longer period of time in cold conditions, the disease spreads mainly in winter months. Although the disease is highly communicable, it is comparatively rare in many developed countries due to vaccination using the attenuated live virus, but occasional outbreaks of CDV infection can still occur in vaccinated populations of dogs. In areas with unvaccinated populations, CD is extremely widespread.

The host spectrum of CDV comprises dogs and many other carnivores and noncarnivores as well as marine mammals. Recently, a possible link between Paget's disease of bone in humans and CDV infection was shown by epidemiological studies and was substantiated by detection of CDV RNA in affected tissues (Gordon, et al., 1992; O’Driscoll, et al., 1990). CDV is also discussed as a candidate that might play a role in the initiation of multiple sclerosis (Rohowsky-Kochan, et al., 1995). Thus prevention of CDV infection in house dogs may have a direct impact on human safety.

Diagnosis of CD in acute or subacute form is usually based on clinical signs and history in unvaccinated puppies. But it has been difficult to differentiate CD from other diseases such as kennel cough in the early stage. Serologic detection of IgM antibody can be useful, but poses a problem in young puppies due to uncertainty caused by maternal antibody interference. Definitive diagnosis can be made through isolation of CDV, or through detection of CDV in epithelial cells after fluorescent antibody (FA) staining. However, virus isolation takes several days to weeks and is frequently not effective in the acute stage of the infection. In addition, FA testing is successful only during the first few days of acute signs of distemper.

CDV detection by PCR is the most rapid, sensitive and specific method for the diagnosis of this infection. It also helps to eliminate false negative and positive cases.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of CDV
  • Early prevention of spread of this virus among a population
  • Minimize human exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Appel, M. J. G., and Gillespie, J.H.(1972). Canine distemper virus, p. 1-96. In S. Gard, C. Hallauer, and K. F. Meyer (ed.), Virology monographs 11. Springer-Verlag, New York, N.Y.
Gordon, M. T., Mee, A.P., Anderson, D.C. and Sharp, P.T. (1992) Canine distemper virus transcripts sequenced from pagetic bone. Bone Miner. 19:159-174.
O'Driscoll, J. B., Buckler, H.M., Jeacock, J. and Anderson, D.C. (1990) Dogs, distemper and osteitis deformans: a further epidemiological study. Bone Miner. 11:209-216.
Rohowsky-Kochan, C., Dowling, P.C., and Cook, S.D. (1995) Canine distemper virus-specific antibodies in multiple sclerosis. Neurology 45:1554-1560.

Specimen requirement: Nasopharyngeal swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml CSF, urine, plasma or serum, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

2003-2017 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880