Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Canine parvovirus PCR test
wildlife and zoo assay data sheet

Canine parvovirus

Test code:
S0091 - Qualitative detection of canine parvovirus by polymerase chain reaction

 

Canine parvovirus (CPV) is a highly contagious viral infection in canids and felines. The virus is transmitted by oral ingestion of viral contaminated feces. Several studies have shown that canine parvovirus and feline panleukopenia virus may undergo mutual interspecies transmission between dogs and cats, and it is postulated that they may cause disease in some adventitious hosts.

The incidence of the disease is highest in young dogs and tends to start some time after the pup has lost its maternal antibody. Any age can be infected but most dogs are infected between the ages of 2 and 6 months when maternal antibody decreases below a protective level. Symptoms are usually mild to nonexistent. However, a full blown case of parvovirus untreated can easily be fatal.

Once inside the body, the virus infects local lymph nodes, quickly multiplies and then via the blood moves to the small intestine where signs of the disease begin in approximately 5-6 days. The virus damages the lining of the small intestine leading to breakdown of crucial disease defense barriers and disturbance of digestive enzyme secretion and nutrient absorption. Additionally, the normal bacterial flora of the small intestine which aid in digestion are now exposed to ulcerated mucosa, providing a direct route into the blood stream. Fluid loss from both vomiting and diarrhea is dramatic and dehydration ensues. The onslaught of bacteria and toxins into the blood will ultimately cause death.

Because of the severity of parvovirus-induced disease and the highly contagious nature of the virus, several assays have been developed to detect the virus in the feces of infected canids. Usually, feces from diarrheic dogs are screened using ELISA or hemagglutination (HA) assays, but these techniques have very low sensitivity. In contrast, methods based on detection of viral DNA by PCR have been shown to be more sensitive and specific.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of canine parvovirus
  • Early prevention of spread of this virus among a population
  • Minimize human exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces, or rectal swab, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative PCR

Normal range: Nondetected

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