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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

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Delftia acidovorans PCR test

wildlife and zoo assay data sheet

Delftia acidovorans

Test code: B0087 - Qualitative ultrasensitive detection of Delftia acidovorans by real time PCR.

Delftia acidovorans was formerly called Comamonas acidovorans or Pseudomonas acidovorans. Prior to 1987, a number of now-independent genera, including Delftia, Burkholderia, Stenotrophomonas, Ralstonia, Comamonas, Acidovorax and others, were all grouped within the broad genus Pseudomonas. In general, these bacteria are aerobic, oxidase-positive, Gram-negative bacilli that grow on MacConkey's agar. When this broad genus was rearranged based on rRNA sequencing data, D. acidovorans was grouped into the family Comamonadaceae based on 16S rRNA gene sequence analysis. However, Comamonas acidovorans was later found to be phylogenetically quite distant from the other species of Comamonas (Wen et al., 1999), and was therefore renamed Delftia acidovorans.

Morphologically, D. acidovorans appears as straight to slightly curved Gram-negative bacilli, occurring singly or in pairs. Strains are motile by means of polar or bipolar tufts of one to six flagella. The organism is strictly aerobic and is a common water and soil saprophyte. D. acidovorans is normally of limited virulence and is usually considered an opportunistic pathogen. Due to its widespread occurrence, it is often implicated in the contamination of medical devices such as intravascular catheters.

Culture differentiation of D. acidovorans can be difficult because it shares many biochemical and growth characteristics with other bacteria in the Pseudomonas genus. Molecular detection by PCR is highly specific and sensitive and can often be used instead of culture to detect and identify D. acidovorans.

Utilities:                      

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Delftia acidovorans infection
  • Help ensure animal groups and populations are free of Delftia acidovorans
  • Help ensure environmental surfaces and devices are free of Delftia acidovorans
  • Early prevention of spread of these bacteria among a population
  • Minimize human exposure to these bacteria
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Wen , A., Fegan, M., Hayward, C., Chakraborty, S. and Sly, L.I. (1999) Phylogenetic relationships among members of the Comamonadaceae, and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov. Int. J. Syst. Bacteriol. 49:567-76.

Specimen requirement:  0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml urine, or environmental swab, or nasopharyngeal swab, or rectal swab, or 0.2 ml feces.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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