Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi


Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever




Coxiella burnetii


Delftia acidovorans

E. coli O157:H7

E. coli panel





Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus



Hepatitis E


Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis



Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia


Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi


Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Edwardsiella PCR test
wildlife, zoo and aquatic assay data sheet


Test code:
- Ultrasensitive qualitative detection of Edwardsiella bacteria by real time PCR. This assay detects but does not differentiate E. tarda, E. ictaluri and E. hoshinae.

Edwardsiella is a genus of gram-negative, facultatively anaerobic, rod-shaped bacteria of the family Enterobacteraceae. They are considered occasional opportunistic pathogens of humans. There are three species in the genus:

1.  Edwardsiella hoshinae has been isolated from animals and humans. It does not produce indole but is motile. E. hoshinae infects birds and reptiles.

2.  Edwardsiella ictaluri is nonmotile and does not produce indole. It usually occurs as a pathogen of catfish.

3.  Edwardsiella tarda is also known as Edwardsiella anguillimortifera. It produces indole, and is biochemically very similar to Escherichia coli. It is usually found in the intestinal tracts of aquatic animals and reptiles. It can also occasionally be isolated from the urine, blood, and feces of humans and has been known to cause gastroenteritis and wound infections. E. tarda is the etiologic agent of edwardsiellosis, a devastating fish disease that affects worldwide aquaculture industries.

These bacteria can be cultured with routine media but identification requires special selective media. PCR enables rapid and highly sensitive detection of these bacteria (Bilodeau et al., 2003).


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Edwardsiella infection
  • Help ensure that animal facilities and populations are free of Edwardsiella
  • Early prevention of spread of these bacteria
  • Minimize human exposure to these bacteria

Bilodeau, A. L., Waldbieser, G.C., Terhune, J.S., Wise, D.J. and & Wolters, W.R. (2003) A Real-Time Polymerase Chain Reaction Assay of the Bacterium Edwardsiella ictaluri in Channel Catfish. J. Aquatic Animal Health 15: 80-86.

Specimen requirements: 0.2 ml of whole blood in EDTA (purple top) or ACD (yellow top) tube, or wound swab, or 0.2 ml feces or urine, or fecal swab, or water sample, or bacterial culture, or food sample

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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