Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

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Citrobacter freundii

Classical swine fever

Clostridium

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Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

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Legionella

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Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

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Q fever

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Reovirus screen

Rickettsia

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Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Edwardsiella PCR test
wildlife, zoo and aquatic assay data sheet

Edwardsiella

Test code:
B0084
- Ultrasensitive qualitative detection of Edwardsiella bacteria by real time PCR. This assay detects but does not differentiate E. tarda, E. ictaluri and E. hoshinae.

Edwardsiella is a genus of gram-negative, facultatively anaerobic, rod-shaped bacteria of the family Enterobacteraceae. They are considered occasional opportunistic pathogens of humans. There are three species in the genus:

1.  Edwardsiella hoshinae has been isolated from animals and humans. It does not produce indole but is motile. E. hoshinae infects birds and reptiles.

2.  Edwardsiella ictaluri is nonmotile and does not produce indole. It usually occurs as a pathogen of catfish.

3.  Edwardsiella tarda is also known as Edwardsiella anguillimortifera. It produces indole, and is biochemically very similar to Escherichia coli. It is usually found in the intestinal tracts of aquatic animals and reptiles. It can also occasionally be isolated from the urine, blood, and feces of humans and has been known to cause gastroenteritis and wound infections. E. tarda is the etiologic agent of edwardsiellosis, a devastating fish disease that affects worldwide aquaculture industries.

These bacteria can be cultured with routine media but identification requires special selective media. PCR enables rapid and highly sensitive detection of these bacteria (Bilodeau et al., 2003).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Edwardsiella infection
  • Help ensure that animal facilities and populations are free of Edwardsiella
  • Early prevention of spread of these bacteria
  • Minimize human exposure to these bacteria

References:
Bilodeau, A. L., Waldbieser, G.C., Terhune, J.S., Wise, D.J. and & Wolters, W.R. (2003) A Real-Time Polymerase Chain Reaction Assay of the Bacterium Edwardsiella ictaluri in Channel Catfish. J. Aquatic Animal Health 15: 80-86.

Specimen requirements: 0.2 ml of whole blood in EDTA (purple top) or ACD (yellow top) tube, or wound swab, or 0.2 ml feces or urine, or fecal swab, or water sample, or bacterial culture, or food sample

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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