For our international clients: Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere. Samples are small, light and stable at room temperature for several weeks.

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Zoologix performs zoo and wildlife tests for...

African swine fever

Aleutian disease

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Classical swine fever

Clostridium difficile

Clostridium screen

Cryptosporidium

E. coli O157:H7

E. coli panel

Ehrlichia risticii

Encephalomyocarditis

Enterovirus

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Giardia

Helicobacter heilmanii

Helicobacter pylori

Hepatitis E

Japanese encephalitis

Klebsiella

Lawsonia intracellularis

Listeria monocytogenes

Lyme disease

Mink enteritis virus

Monkeypox

Mycobacteria

Mycoplasma pneumoniae

Mycoplasma screen

Neospora caninum

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Potomac horse fever

Pseudorabies

Rabies

Reovirus screen

Rotavirus screen

Salmonella

Sarcocystis neurona

St. Louis encephalitis

Strep pneumoniae

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trypanosoma cruzi

Trypanosoma evansi

Vesicular stomatitis

West Nile virus

Yersinia pseudotuberculosis

...and more -- see our assay menu for a complete listing of zoo and wildlife assays.


wildlife and zoo assay data sheet

Helicobacter heilmannii

Test codes:

B0023 - Ultrasensitive qualitative detection of Helicobacter heilmannii by real time polymerase chain reaction
P0010 - Ultrasensitive Helicobacter species screen by nested polymerase chain reaction
P0011 - Ultrasensitive Helicobacter species identification by nested polymerase chain reaction and restriction fragment length polymorphism

Helicobacter heilmannii (previously known as Gastrospirillum hominis) is a 4-10 Ám long, spiral-shaped, motile bacterium with three to eight coils, a wavelength of about 1 Ám, up to 14 uni- or bipolar flagella, and no periplasmic filaments. Gastric infection with Helicobacter heilmannii is associated with the development of chronic gastritis (found in the stomachs of 0.2 to 4% of patients with gastritis) and low-grade mucosa-associated lymphoid tissue lymphoma in humans. Eradication of H. heilmannii by antibiotic treatment of patients can result in complete remission of MALT lymphoma, indicating a causal relationship between H. heilmannii infection and MALT lymphoma. Unlike H. pylori infections, gastric infections with H. heilmannii or Gastrospirillum-like organisms are not restricted to humans. A broad range of animals, including dogs, cats, pigs, and cattle, are naturally infected, with frequencies ranging from 80% to 100%. It has been suggested that H. heilmannii infection in humans is a zoonosis and that animals serve as a reservoir for transmission to humans.

Definitive culture of H. heilmannii has not been achieved to date (Anderson et al., 1996) and diagnosis of H. heilmannii infection is usually made on the basis of its distinct spiral morphology, compared with H. pylori, on silver- stained tissue sections. However, there are a number large gastric spiral organisms such as H. felis, H. salomonis, and H. bizzozeronii are indistinguishable from H. heilmannii on routine light microscopy, and H. pylori grown in a broth culture can also adopt a morphology identical to that of H. heilmannii (Fawcett et al., 1999). Molecular detection methods, such as PCR, are always required for more definitive identification (Trebesius et al., 2001).

Utilities:

  • Confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of H. heilmannii infection
  • Ensure that animal colonies are free of H. heilmannii
  • Early prevention of spread of this bacterium among a colony
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products and vaccines that derive from animals

References:
Andersen, L.P., Norgaard, A., Holck, S., Blom, J. and Elsborg, L. (1996) Isolation of a "Helicobacter heilmannii"-like organism from the human stomach. Eur. J. Clin. Microbiol. Infect. Dis. 15:95-96.
Fawcett, P.T., Gibney, K.M. and Vinette, K.M. (1999) Helicobacter pylori can be induced to assume the morphology of Helicobacter heilmannii. J. Clin. Microbiol. 37:1045-1048.
Trebesius, K., Adler, K., Vieth, M., Stolte, M. and Haas, R. (2001) Specific detection and prevalence of Helicobacter heilmannii-like organisms in the human gastric mucosa by fluorescent in situ hybridization and partial 16S ribosomal DNA sequencing. J. Clin. Microbiol. 39:1510-1516.

Specimen requirement: 1 ml gastric lavage or feces or tissue shipped overnight at room temperature; or tissue shipped frozen.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodologies: Qualitative real time PCR

Normal range: Nondetected

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