Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Hepatitis E PCR test
wildlife and zoo assay data sheet

Hepatitis E

Test code:
S0123 - Ultrasensitive qualitative detection of hepatitis E virus by reverse transcription coupled real time polymerase chain reaction

 

Hepatitis E virus (HEV) is transmitted by the fecal-oral route and the disease is usually self-limiting. It is often spread by fecally contaminated water within endemic areas. It has many similarities with hepatitis A.

Because of similar physicochemical and biological properties, HEV is classified in the Caliciviridae family. However, recent molecular study has shown that the HEV genome is different from the other caliciviruses, and suggests that its genomic sequences are more similar to those of rubella virus. Therefore, HEV eventually may be reclassified.

Domestic animals have been reported as a reservoir for the hepatitis E virus, with some surveys showing infection rates exceeding 95% among domestic pigs (Satou and Nishiura, 2007). Consumption of wild boar meat and uncooked deer meat has been reported to transmit the virus. Rats also carry the virus.

Zoonotic transmission of this virus from pet pigs to owners is a growing concern. Pigs also may be a source of HEV infection of humans through xenotransplantation of pig tissues or organs, such as liver, pancreas, and heart, to humans.

Detection of HEV infection by bioassay or serological methods is labor intensive and has low sensitivity. Molecular detection by PCR is a rapid, specific and sensitive alternative (Lin et al. 2000).

Utilities:

  • Help confirm the disease causing agent
  • Identify HEV carriers
  • Help ensure that animal groups and populations are free of HEV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Lin, C.C., Wu, J.C., Chang, T.T., Chang, W.Y., Yu, M.L., Tam, A.W., Wang, S.C., Huang, Y.H., Chang, F.Y., and Lee, S.D. (2000) Diagnostic value of immunoglobulin G (IgG) and IgM anti-hepatitis E virus (HEV) tests based on HEV RNA in an area where hepatitis E is not endemic. J. Clin. Microbiol. 38:3915-3918.

Satou, K. and Nishiura, H (2007) Transmission dynamics of hepatitis E among swine: potential impact upon human infection. BMC Vet. Res. 3:9

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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