wildlife and zoo assay data sheet
Ultrasensitive qualitative detection of hepatitis E virus by
reverse transcription coupled real time polymerase chain
virus (HEV) is transmitted by the fecal-oral route and the
disease is usually self-limiting. It is often spread by fecally
contaminated water within endemic areas. It has many
similarities with hepatitis A.
similar physicochemical and biological properties, HEV is
classified in the Caliciviridae family. However, recent
molecular study has shown that the HEV genome is different from
the other caliciviruses, and suggests that its genomic sequences
are more similar to those of rubella virus. Therefore, HEV
eventually may be reclassified.
animals have been reported as a reservoir for the hepatitis E
virus, with some surveys showing infection rates exceeding 95%
among domestic pigs (Satou and Nishiura, 2007). Consumption of
wild boar meat and uncooked deer meat has been reported to
transmit the virus. Rats also carry the virus.
transmission of this virus from pet pigs to owners is a growing
concern. Pigs also may be a source of HEV infection of humans
through xenotransplantation of pig tissues or organs, such as
liver, pancreas, and heart, to humans.
HEV infection by bioassay or serological methods is labor
intensive and has low sensitivity. Molecular detection by PCR is
a rapid, specific and sensitive alternative (Lin et al. 2000).
Help confirm the disease causing agent
Identify HEV carriers
Help ensure that animal groups and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
Lin, C.C., Wu, J.C., Chang, T.T., Chang, W.Y., Yu, M.L., Tam,
A.W., Wang, S.C., Huang, Y.H., Chang, F.Y., and Lee, S.D. (2000)
Diagnostic value of immunoglobulin G (IgG) and IgM
anti-hepatitis E virus (HEV) tests based on HEV RNA in an area
where hepatitis E is not endemic. J. Clin. Microbiol.
and Nishiura, H (2007) Transmission dynamics of hepatitis E
among swine: potential impact upon human infection. BMC Vet.
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription coupled real time PCR