Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms

Aspergillus

Babesia

Bacillus species

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Camelpox

Campylobacter

Canine circovirus

Canine distemper

Canine parvovirus

Capillaria xenopodis

Chlamydia/
Chlamydophila

Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptococcosis

Cryptosporidium

Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacter cloacae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Francisella tularensis

Giardia

Hantavirus

Helicobacter

Hepatitis E

Herring worms

Histoplasma

Inclusion Body Disease (IBD)

Influenza type A

Influenza type B

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses

Malaria

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Pentastomid worms

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia

Pseudorabies

Pseudoterranova worms

Q fever

Rabies

Raillietiella orientalis

Ranavirus

Reovirus screen

Reptarenavirus

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Tongue worms

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Tularemia

Turtle fraservirus

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Hepatitis E PCR test
wildlife and zoo assay data sheet

Hepatitis E

Test code:
S0123 - Ultrasensitive qualitative detection of hepatitis E virus by reverse transcription coupled real time polymerase chain reaction

 

Hepatitis E virus (HEV) is transmitted by the fecal-oral route and the disease is usually self-limiting. It is often spread by fecally contaminated water within endemic areas. It has many similarities with hepatitis A.

Because of similar physicochemical and biological properties, HEV is classified in the Caliciviridae family. However, recent molecular study has shown that the HEV genome is different from the other caliciviruses, and suggests that its genomic sequences are more similar to those of rubella virus. Therefore, HEV eventually may be reclassified.

Domestic animals have been reported as a reservoir for the hepatitis E virus, with some surveys showing infection rates exceeding 95% among domestic pigs (Satou and Nishiura, 2007). Consumption of wild boar meat and uncooked deer meat has been reported to transmit the virus. Rats also carry the virus.

Zoonotic transmission of this virus from pet pigs to owners is a growing concern. Pigs also may be a source of HEV infection of humans through xenotransplantation of pig tissues or organs, such as liver, pancreas, and heart, to humans.

Detection of HEV infection by bioassay or serological methods is labor intensive and has low sensitivity. Molecular detection by PCR is a rapid, specific and sensitive alternative (Lin et al. 2000).

Utilities:

  • Help confirm the disease causing agent
  • Identify HEV carriers
  • Help ensure that animal groups and populations are free of HEV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Lin, C.C., Wu, J.C., Chang, T.T., Chang, W.Y., Yu, M.L., Tam, A.W., Wang, S.C., Huang, Y.H., Chang, F.Y., and Lee, S.D. (2000) Diagnostic value of immunoglobulin G (IgG) and IgM anti-hepatitis E virus (HEV) tests based on HEV RNA in an area where hepatitis E is not endemic. J. Clin. Microbiol. 38:3915-3918.

Satou, K. and Nishiura, H (2007) Transmission dynamics of hepatitis E among swine: potential impact upon human infection. BMC Vet. Res. 3:9

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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