Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

* * *

Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi



Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii


Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel





Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus




Hepatitis E


Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia


Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi


Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Histoplasma PCR test

wildlife and zoo assay data sheet


Test code:
F0006 - Ultrasensitive qualitative detection of Histoplasma species by real time PCR

Histoplasma is a genus of dimorphic fungi belonging to the family Ajellomycetaceae which are commonly found in bird and bat fecal material. The Histoplasma genus includes H. capsulatum which causes histoplasmosis; H. farciminosum which causes epizootic lymphangitis in horses; and H. duboisii which causes African histoplasmosis.

H. capsulatum is most prevalent in the Ohio and Mississippi river valleys. The disease caused by H. capsulatum is called histoplasmosis, also known as "cave disease," "Darling's disease," "Ohio Valley disease," "Reticuloendotheliosis," "spelunker’s lung" and “caver's disease.” Symptoms primarily affect the lungs, but other organs can be affected if the fungus spreads in the body. Histoplasmosis is commonly found in immunocompromised individuals such as AIDS patients and cancer patients.

People can be infected by inhaling microscopic fungi borne from bird or bat feces, or decomposing human biological fluids including urine, vomit, and feces.

Histoplasmosis can be diagnosed by detection of antigens in blood or urine samples by immunological or molecular methods. However, immunology is not very specific because antigens of Histoplasma can cross-react with antigens of blastomycosis, coccidioidomycosis, paracoccidioidomycosis and Penicillium marneffei infection. Formal histoplasmosis diagnoses may be confirmed by culturing the fungus directly. However, cultures may take up to 6 weeks for diagnostic growth to occur. Molecular detection by polymerase chain reaction (PCR) can provide a rapid, specific and sensitive method for diagnosis of this fungus (Elias et al., 2012).


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Histoplasma infection
  • Help ensure that amphibian colonies are free of Histoplasma
  • Early prevention of spread of this fungus among the colony
  • Environmental monitoring for this fungus
  • Minimize human exposure to this fungus
  • Safety monitoring of biological products that derive from susceptible species

Elías, N.A., Cuestas, M.L., Sandoval, M., Poblete, G., Lopez-Daneri, G., Jewtuchowicz, V., Iovannitti, C. and Mujica, M.T. (2012) Rapid identification of Histoplasma capsulatum directly from cultures by multiplex PCR. Mycopathologia. 174:451-456.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or urine, or sputum, or bronchoalveolar lavage, or feces or fecal swab, or 0.2 ml fresh, frozen or fixed tissue, or environmental surface swab.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Real time polymerase chain reaction

Normal range: Nondetected

©2003-2018 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880