Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

* * *

Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Lawsonia intracellularis PCR test
wildlife and zoo assay data sheet

Lawsonia intracellularis

Test code:
B0035 - Ultrasensitive qualitative detection of Lawsonia intracellularis by real time polymerase chain reaction

 

Proliferative enteropathy, also known as proliferative ileitis, is caused by infection with Lawsonia intracellularis, an obligate intracellular, curve-shaped, argyrophilic bacterium. The disease has been detected in domestic and laboratory animals including primates, pig, horse, dog, rat, ferret, guinea pig, rabbit and hamster. The disease has been reported sporadically in foals 3-7 months of age and one outbreak involving 3 different breeding farms has been described. All reported cases were in the eastern half of Canada or the United States. The swine industry suffers the most significant impact from this disease. The disease has two clinical manifestations in pigs: an acute hemorrhagic form often called porcine hemorrhagic enteropathy, and a more chronic proliferative form often referred as porcine intestinal adenomatosis.

Environmental contamination with feces of infected animals appears to be the most important route of transmission of disease, but it is currently unknown how long Lawsonia intracellularis can remain infectious outside the animal. Infection occurs most often during the post-weaning period, when passive maternal immunity declines. After ingestion, the bacteria infect intestinal proliferating crypt epithelial cells and multiply within the apical cytoplasm. There is no evidence of infection of tissues other than intestine. Most infected animals have subclinical infection but shed the bacteria in their feces, leading to environmental contamination. Clinical manifestation of an infection can be triggered by stressors, such as overcrowding, transport, change in diet, and experimental manipulation.

Bacterial culture and isolation of the bacteria are difficult because cultured enterocytes are required to support the growth of Lawsonia intracellularis. Electron microscopy can be used to detect the curved bacterial rods in apical cytoplasm of enterocytes but this is a very time-consuming process and few laboratories have such capability. Immunohistochemical detection can be performed on formalin-fixed, paraffin-embedded tissue from affected intestine to detect the bacteria in following biopsy or necropsy, but this is also a very time-consuming process. Molecular detection using PCR is the most sensitive, rapid and specific method to confirm presence of the Lawsonia intracellularis genome within tissue samples (Jones et al., 1993).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Lawsonia infection
  • Help ensure that animal colonies are free of Lawsonia
  • Early prevention of spread of this bacterium among a colony
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products and vaccines that derive from animals

References:
Jones, G. F., Ward, G.E., Murtaugh, M.P., Lin, G. and Gebhart, C.J. (1993) Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis in feces by polymerase chain reaction. J. Clin. Microbiol. 31:2611-2615.

Specimen requirement: 0.2 ml feces, or rectal swab, or 0.2 ml fresh, frozen or fixed ileum tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2017 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880