Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Leptospira PCR test

wildlife and zoo assay data sheet

Leptospira

Test code:
B0051 - Ultrasensitive qualitative detection of Leptospira bacteria by real time polymerase chain reaction. This test detects but does not differentiate common Leptospira serovars.

 

Leptospirosis is a disease of worldwide significance that infects many domestic and wildlife animal species and humans. The scientific name of the infecting organism is Leptospira interrogans sensu lato. Within this species there are many different strains (serovars).

Leptospira can be spread between animals through contact with infected urine, venereal or placental transfer, or bite wounds. Indirect transmission occurs through exposure of susceptible animals to contaminated water sources, food, bedding or human hands. Leptospira bacteria can survive in feces up to 2 months. Stagnant or slow moving water provides a suitable habitat; the organism can survive up to 20 days in water. This explains why outbreaks often increase during periods of flooding.

Fever, shivering and muscle tenderness are among the first signs of acute infection. Rapid dehydration may develop subsequently. In subacute infections, the animal usually develops a fever, anorexia, dehydration, and increased thirst. Animals with liver involvement may develop icterus. Conjunctivitis and recurrent uveitis may occur in chronic infections. Breeding success can be impacted because of increased risk of abortion and decreased milk production.

Although Leptospira infection can be diagnosed by change in antibody titers, titers may be negative during early stages of critical infection. Thus an alternative method is needed to provide a quick diagnosis of the disease at this stage. Molecular detection of PCR is rapid, highly sensitive and specific. It is suitable for quick diagnosis even during the first few days of infection.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Leptospira infection.
  • Help ensure that animal populations are free of Leptospira
  • Early prevention of spread of this bacterium
  • Minimize personnel exposure to this bacterium
  • Safety monitoring of biological products that derive from susceptible animals

Specimen requirements: Urogenital swab, or cervical swab, or 0.2 ml urine, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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