Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Listeria monocytogenes PCR test
wildlife and zoo assay data sheet

Listeria monocytogenes

Test code:
B0044 - Ultrasensitive qualitative detection of Listeria monocytogenes bacteria by real time polymerase chain reaction. Assay does not detect other Listeria species.

 

Bacteria of the genus Listeria are widely distributed in the environment and are a common contaminant of food. Listeria monocytogenes is of particular concern, as it is the major culprit of listeriosis. Food products most frequently associated with listeriosis are soft cheeses, particularly those made from unpasteurized milk, and ready-to-eat animal and human food products containing meat.

Infection with L. monocytogenes can result in septicemia, meningoencephalitis and abortion in humans and animals, primarily affecting pregnant, newborn and immunocompromised individuals.

L. monocytogenes is a non-sporogenic, facultative intracellular gram-positive bacterium that has the ability to survive high and low temperatures, low pH and high salinity. It is not acid-resistant and can be grown on a tryptose and blood agar.

Traditionally, culture-based identification of Listeria from food, fecal or environmental samples relied on selective enrichments and subsequent biochemical analyses. This analytical approach is laborious and time-consuming (Almeida and Almeida, 2000), and is prone to contamination during handling and incubation.

Detection of this pathogen by polymerase chain reaction is highly sensitive and specific. The assay is performed more quickly than culture, on a wide range of specimen types, including environmental samples, with a high degree of accuracy.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Listeriosis
  • Safety monitoring of food products and processing facilities
  • Minimize human and animal exposure to L. monocytogenes

References:
Almeida, P.F., Almeida, R.C.C. 2000: A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes. Food Control II: 97-101

Specimen requirement: Food swab, or environmental surface swab, or rectal swab, or 0.2 ml feces.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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