Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Batrachochytrium salamandrivorans

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Macropod herpesvirus PCR test for kangaroos
wildlife and zoo assay data sheet

Macropodid (kangaroo) herpesviruses

Test codes:
S0161
- Ultrasensitive qualitative detection but not differentiation of macropod herpesviruses 1 and 2 by real time polymerase chain reaction.
S0162 - Ultrasensitive qualitative detection of macropod herpesvirus 3 by real time polymerase chain reaction.

Macropodid herpesviruses (MaHVs) have caused fatal disease outbreaks among captive marsupials in Australia. These outbreaks resulted in the isolation of nine MaHVs, grouped into two species, macropodid herpesviruses 1 and 2 (MaHV-1 and MaHV-2). Serological surveys show that these viruses are widespread among Australian kangaroos and wallabies (family Macropodidae).

Phylogenetic reconstructions based on putative amino acid sequences of glycoprotein B indicate that MaHV-1 and -2 are most closely related to subfamily Alphaherpesvirinae. Within this subfamily, MaHV-1 and -2 are closely associated with herpesviruses that infect primates.

Recently a new species of macropodid herpesvirus, MaHV-3, has been discovered in a group of free-ranging eastern grey kangaroos (Wilcox et al., 2011). This virus is more closely related to the subfamily Gammaherpesvirinae than to MaHV-1 and MaHV-2

Infections of macropodids by any of these three viruses can result in congestion of lung, liver, and spleen. Infected animals may have high fever, respiratory rales, conjunctivitis, and small vesicles in the ano-genital region. Similar to herpesvirus infection in humans, infected animals may subsequently develop latent infections with subclinical symptoms. These animals become carriers.

These viruses can be detected by cell culture, but this method is expensive and time consuming. Since many macropods have had past exposure to these viruses, serological detection is not very useful to identify carriers. Molecular detection by polymerase chain reaction offers rapid, highly specific and sensitive detection of these viruses.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of these viruses
  • Early prevention of spread of these viruses among a population
  • Minimize human exposure to these viruses

References:
Wilcox, R.S., Vaz, P., Ficorilli, N.P., Whiteley, P.L., Wilks, C.R. and Devlin, J.M. (2011) Gammaherpesvirus infection in a free-ranging eastern grey kangaroo (Macropus giganteus). Aust. Vet. J. 89:55-57.

Specimen requirements:  0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasal swab, or throat swab, or lesion swab, or 0.2 ml cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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