Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Porcine cytomegalovirus PCR test
wildlife and zoo assay data sheet

Porcine cytomegalovirus

Test code:
S0128 - Ultrasensitive qualitative detection of porcine cytomegalovirus by real time PCR

 

Herpesviruses are widely distributed and have been found in insects, reptiles, amphibians and every species of bird and mammal. One important characteristic of herpesvirus infection is that the virus persists in the infected host for life and is frequently reactivated and shed. In pigs, five herpesviruses have been identified: three recently identified lymphotrophic herpesviruses, pseudorabies virus and porcine cytomegalovirus (PCMV).

PCMV causes inclusion body rhinitis and abortion or neonatal piglet losses in pigs. On microscopic examination, CMV infection causes large intranuclear inclusion bodies in infected cells. In pigs, a major site of infection tends to be the turbinates and the rest of the upper respiratory tract. Clinically, inclusion body rhinitis is often confused with atrophic rhinitis, another upper respiratory tract disease of multiple etiologies.

Like human CMV, porcine CMV crosses the placenta and infects fetuses, with resulting congenital infections. In susceptible herds, infection with PCMV can lead to fetal and piglet death, runting, rhinitis, pneumonia, and poor weight gain. In herds where management conditions tend to be good or exceptional, the virus may be endemic without causing any apparent clinical disease or economic loss. However, these infected animals can be latent carriers of the virus.

Antibodies to this virus have been found in a high percentage of swine herds worldwide. Because of the high prevalence of positive serology, serological identification of infected pigs is not useful. Many latent carriers remain unidentified, posing serious problems with research using the pig as a model. In xenotransplantation between pig and human, reactivation of the latent virus can cause postransplantation failure. Molecular detection of the virus is an important tool that can provide rapid, sensitive and specific detection of the viral nucleic acid in suspected animals (Hamel et al., 1999).

Utilities:

  • Help confirm the disease causing agent
  • Identify PCMV carriers
  • Help ensure that animal colonies and populations are free of PCMV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Hamel, A.L., Lin, L., Sachvie, C., Grudeski, E., and Nayar, G.P.S. (1999) Assay for Detecting Porcine Cytomegalovirus. J Clin Microbiol. 37: 3767–3768.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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