Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Porcine lymphotropic herpesvirus PCR test
wildlife and zoo assay data sheet

Porcine lymphotropic herpesviruses 1 and 2

Test code:
S0129 - Ultrasensitive qualitative detection of porcine lymphotropic herpesviruses 1 and 2 by real time PCR. Assay detects but does not differentiate PLHV-1 and PLHV-2. Assay does not detect PLHV-3.

 

Herpesviruses are widely distributed and have been found in insects, reptiles, amphibians and every species of bird and mammal. One important characteristic of herpesvirus infection is that the virus persists in the infected host for life and is frequently reactivated and shed. In pigs, five herpesviruses have been identified: pseudorabies virus, porcine cytomegalovirus (PCMV) and three recently identified lymphotrophic herpesviruses, PLHV-1, PLHV-2 and PLHV-3.

PLHV-1 and -2 are highly homologous to each other but not to PLHV-3. The two viruses are widespread in domestic pigs and are closely related to several ruminant gammaherpesviruses, most of which are etiologically implicated in the occurrence of malignant catarrhal fever (MCF), a lymphoproliferative inflammatory disease with an often fatal outcome. PLHV-1 and -2 are also related to Epstein-Barr Virus, Human Herpesvirus-8 and other gammaherpesviruses.

A recent study (McMahon et al., 2006) in domestic pigs has shown that PLHV1 infections are most common, being found in 74% of animals tested, followed by PLHV3 at 45% and PLHV2 at 21%. Infections with multiple PLHV species were frequently detected.

Like porcine CMV, antibodies to PLHV have been found in a high percentage of swine herds worldwide. Because of the high prevalence of positive serology, serological identification of infected pigs is not possible. Many of these latent carriers remain unidentified, posing serious problems for research using the pig as a model. In xenotransplantation between pig and human, reactivation of these latent viruses can cause postransplantation failure. Molecular detection of these viruses is an important tool to provide rapid, sensitive and specific detection of the viral nucleic acid in suspected animals.

Utilities:

  • Help confirm the disease causing agent
  • Identify PLHV-1 and -2 carriers
  • Help ensure that animal colonies and populations are free of PLHV-1 and -2
  • Early prevention of spread of these viruses among animals
  • Minimize human exposure to these viruses
  • Safety monitoring of biological products that derive from animals

References:
McMahon, K.J., Minihan, D., Campion, E.M., Loughran, S.T., Allan, G., McNeilly, F. and Walls, D. (2006) Infection of pigs in Ireland with lymphotropic ?-herpesviruses and relationship to postweaning multisystemic wasting syndrome. Vet. Microbiol. 116:60-68.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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