Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

* * *

Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi



Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii


Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel





Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus




Hepatitis E


Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia


Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi


Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Pseudoloma neurophilia PCR test

wildlife, zoo and aquatic assay data sheet

Pseudoloma neurophilia

Test code:
X0027 - Ultrasensitive qualitative detection of Pseudoloma neurophilia by real time PCR.

Pseudoloma neurophilia, a microsporidian parasite, is the most prevalent pathogen found in zebrafish (Danio rerio) research facilities. Infections occur primarily in the spinal cord and are associated with emaciation and scoliotic changes. It was initially discovered as an unusual xenoma-forming microsporidium in the central nervous system of moribund zebrafish from a laboratory colony in Eugene, Oregon.

Spores of this parasite are uninucleate, ovoid to pyriform, with a prominent posterior vacuole. The spores are average 5.4 2.7 μm in size with 13–16 coils of the polar filament. Infection with this parasite can produce xenomas within the spinal cord and hindbrain. The xenomas usually contain sporophorous vesicles with up to 16 spores. Sporoblasts and presporoblast stages (probably sporonts) are found occasionally in small aggregates dispersed randomly throughout the xenomas.

This parasite can also be found in the ovaries of infected fishes. Vertical transmission appears to help spread the parasite through a population. The sharing of various strains of zebrafish among research facilities can spread the parasites to other research facilities.

Compared with traditional diagnostic methods, PCR diagnosis is rapid, specific and highly sensitive. It allows for screening of large numbers of fish, and can be applied to eggs, water filtrates, biofilms, and other sample types (Whipps and Kent, 2006.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of this parasite
  • Help ensure that zebrafish facilities are free of this parasite
  • Early prevention of spread of this parasite in a facility or geographic area
  • Minimize human exposure to this parasite
  • Safety monitoring of biological products that derive from susceptible animals

Whipps, C.M. and Kent, M.L. (2006) Polymerase Chain Reaction Detection of Pseudoloma neurophilia, a Common Microsporidian of Zebrafish (Danio rerio) Reared in Research Laboratories. J. Am. Assoc. Lab. Anim. Sci. 45: 36–39.

Specimen requirements:  Skin or vent swab, or biofilm swab, or filter media, or 10 ml water, or 0.2 ml tissue, or 0.2 ml culture.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2018 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880