Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Pseudorabies PCR test
wildlife and zoo assay data sheet

Pseudorabies

Test code:
S0121 - Ultrasensitive qualitative detection of pseudorabies virus by real time polymerase chain reaction

 

Pseudorabies is a highly endemic swine disease in most parts of the world. It is caused by porcine herpesvirus 1, which is also called pseudorabies virus (PRV), suid herpesvirus-1 (SHV-1), or Aujeszky's disease virus. In cattle, it is sometimes called mad itch disease virus. PRV is considered the most economically important viral disease of swine in areas where hog cholera has been eradicated.

There are many secondary hosts of pseudorabies, including dogs, cats, cattle, rats and horses, but not humans. Secondary hosts are infected through direct contact with swine, rats, and other infected secondary hosts and by eating infected uncooked pork.

Infected swine are usually asymptomatic, but PRV can cause abortion and high mortality in piglets. In some piglets and mature pigs, coughing, sneezing, fever, constipation, depression, seizures, ataxia, circling, and excess salivation can be observed. Mortality in piglets less than one month of age is close to 100 percent, but it is less than 10 percent in pigs between one and six months of age. In cattle infected with this virus, intense itching can result, followed by neurological signs and death. In dogs, symptoms include intense itching, jaw and pharyngeal paralysis, howling and death. In cats, the disease is so rapidly fatal that there are usually no symptoms.

Although the virus does not infect humans, this virus could pose significant risk to xenotransplantation of organs derived from pigs. Studies in experimental animals have shown that the virus can rapidly spread in the central nervous system.

Detection of the virus can be performed by serology assays, but false positive results have been reported (Jacobs et al., 1999). Molecular detection of viral DNA by PCR is a useful alternative because the method is rapid, sensitive and specific.

Utilities:

  • Help confirm the disease causing agent
  • Identify pseudorabies carriers
  • Help ensure that animal groups and populations are free of pseudorabies virus
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Jacobs, L., Voets, R. and Bianchi, A.T.J. (1999) Detection of pseudorabies virus DNA in individual single-reactor pigs found in certified pseudorabies-free herds. Res. Vet. Sci. 67: 305-307.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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