Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Rabies PCR test
wildlife and zoo assay data sheet

Rabies

Test code:
S0116 - Ultrasensitive qualitative detection of rabies virus by reverse transcription coupled real time polymerase chain reaction

 

Rabies virus, a nonsegmented, negative-stranded RNA virus, is a member of the Rhabdoviridae family. This family includes at least three genera of animal viruses, Lyssavirus, Ephemerovirus, and Vesiculovirus. The genus Lyssavirus includes rabies virus, Lagos bat virus, Mokola virus, Duvenhage virus, European bat virus 1 & 2 and Australian bat virus.

Rabies virus can cause fatal acute encephalitis in all mammalian hosts, including humans, dogs, cats, ferrets, pigs, livestock and many other species. However, only a few species are important as reservoirs for the disease. In the United States, several distinct rabies virus variants have been identified in raccoons, skunks, foxes, coyotes and several species of insectivorous bats.

Transmission of rabies virus usually begins when an uninfected animal contacts the saliva of an infected host animal. Various routes of transmission have been documented, including contamination of mucous membranes (ie eyes, nose, and mouth) and even aerosol transmission. However, the most common mode of rabies virus transmission is a bite from an infected host animal.

Initial symptoms of rabies infection in animals include lethargy, fever, vomiting, and anorexia. Signs progress within days to cerebral dysfunction, ataxia, weakness, paralysis, seizures, difficulty breathing, difficulty swallowing, excessive salivation, abnormal behavior, aggression, and/or self-mutilation.

Serology testing has been used to diagnose rabies virus exposure in animals. Direct fluorescent antibody testing is most frequently used to diagnose rabies. This test requires brain tissue from the animal suspected of being rabid. The test can only be performed post-mortem and is not suitable for testing live animals that may have contacted the virus. However, since animals may have had prior exposure to the virus, serology testing may not be specific in confirming the current presence of the virus. An extensive and time-consuming serology titering study may be required to prove the animal’s recent exposure.

Molecular detection by PCR is a rapid, sensitive and specific method to identify the presence of the rabies virus in a sample. The PCR test can be performed on saliva, spinal fluid or a bite lesion swab or biopsy.

See the US Centers for Disease Control (CDC) rabies website at http://www.cdc.gov/rabies/ for additional information on the diagnosis of rabies.

Utilities:

  • Help confirm the disease causing agent
  • Identify rabies carriers
  • Help ensure that animal groups and populations are free of rabies virus
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
http://www.cdc.gov/rabies/ (Centers for Disease Control and Prevention’s rabies information website)

Specimen requirements: Buccal swab, or 0.2 ml CSF, or 0.2 ml fresh or frozen brain stem tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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