Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Ranavirus PCR test

wildlife and zoo assay data sheet

Ranavirus screen

Test code:
S0145 - Ultrasensitive qualitative detection of ranavirus by real time PCR.

S0145 is included in P0031 - amphibian screening panel

Ranaviruses are large viruses with linear and double-stranded DNAs, icosahedral capsids and lipid membranes. They replicate in the nucleus and cytoplasm of host cells.

Ranaviruses can infect fish, amphibians and other vertebrates (Chinchar, 2002). Infection of larval salamanders with these viruses results in high mortality rates. Ranavirus infection usually occurs in late summer or early autumn, and recurs annually in some locations.

Amphibians infected with Ranaviruses develop generalized viremia resulting in edema, papules, lesions, and bloody exudate from the vent. Necrosis of the liver, spleen, kidney and hematopoietic tissues may result in death after 7–14 days of infection.

Viral transmission between animals can occur through close physical contact, contact with infected water or ingestion of infected carcasses. Vertical transmission of virus from infected adults to eggs is unknown, and the virus does not appear to have a reservoir host.

Serological detection and culture detection of these viruses are both time-consuming and not very sensitive. Molecular detection of the viruses by PCR can be useful because it provides high sensitivity, high specificity and fast turnaround time.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of ranavirus
  • Help ensure that animal facilities or amphibian populations are free of ranaviruses
  • Early prevention of spread of ranaviruses in a facility or geographic area
  • Minimize human exposure to ranaviruses
  • Safety monitoring of biological products that derive from susceptible animals

References:
Chinchar, V.G. (2002) Ranaviruses (family Iridoviridae): Emerging cold-blooded killers—Brief review. Arch. of Virol. 147: 447–470.

Specimen requirements:  Skin or vent swab, or environmental swab, or 0.2 ml water, tissue or culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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