Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Reovirus PCR test
wildlife and zoo assay data sheet

Reovirus screen

Test code S0120  - Ultrasensitive qualitative screen for mammalian reoviruses by reverse transcription coupled real time PCR.  This assay detects a wide range of mammalian reoviruses but does not detect avian reovirus strains.

 

Reoviruses are comprised of 10 to 12 double-stranded RNA genomic segments that can reassort both in nature and in laboratory settings. The most common mammalian isolates are type 1 (Lang), type 2 (Jones), and type 3 (Dearing).

Reoviruses have a high endemic infection rate in many mammals, such as primates, cattle, cats, dogs, rodents and swine. These viruses are common in raw water sources and are often found along with other animal viruses. In humans, the viruses cause only asymptomatic or mild respiratory infections. However, research suggests that reoviruses may be associated with potentially more severe illnesses. Reoviruses have been linked to neonatal hepatitis, extrahepatic biliary atresia, meningitis and myocarditis. Also, immunocompromised, young and elderly individuals may become susceptible to severe bacterial respiratory disease due to an initial reovirus infection.

Due to their widespread occurrence and the ability of these viruses to survive a long period of time in the environment, contamination of water sources has been frequently reported. Animals are especially prone to infection by these viruses. Xenotransplantation of animal organs is severely endangered by potential contamination with these viruses.

Diagnosis of reovirus infection by nonmolecular means is very difficult and is usually based on virus isolation on cell cultures and electron microscopy. These methods are not very sensitive (Muscillo et al., 2001) and are likely underestimate the presence of these viruses in animals and humans. Molecular detection by PCR is the most sensitive, rapid and specific method for identifying reoviruses.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of reoviruses
  • Early prevention of spread of reoviruses among a population
  • Minimize human exposure to reoviruses
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Muscillo M., La Rosa G., Marianelli C., Zaniratti S., Capobianchi M.R., Cantiani L. and Carducci A. (2001) A new RT-PCR method for the identification of reoviruses in seawater samples, Water Res. 35:548–556.

Specimen requirements: Tracheal swab, nasal swab or rectal swab, or 0.2 ml feces, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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