Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Salmonella PCR test
environmental, wildlife and zoo assay data sheet

Salmonella

Test code:
B0040 - Qualitative ultrasensitive detection of Salmonella bacteria by real time polymerase chain reaction.  This assay detects but does not differentiate most recognized serotypes of Salmonella bacteria.

Test B0040 is included in P0041 - waterborne pathogens screening panel and in P0047 - ruminant fecal screening panel

Salmonella are gram negative bacteria that occur worldwide, inhabiting the intestinal tracts of many species including humans, nonhuman primates, birds, horses, pigs, dogs, cats, rats, mice, hamsters, guinea pigs and other species. The house mouse is of special concern, as it is often a carrier and plays a significant role in spreading human and animal salmonellosis.

There are more than 1600 recognized serotypes of Salmonella. According to a recent report (http://www.defra.gov.uk/corporate/vla/science/ documents/science-end-survrep-qtlyw405.pdf), garden bird salmonellosis is the most frequently diagnosed infectious disease among the wild birds submitted to the United Kingdom’s Diseases of Wildlife scheme. The condition is primarily caused by infection with Salmonella typhimurium, although other serotypes have been reported. Environmental contamination by these infected birds can lead to the exposure of other species, notably cats, horses and humans. Animal feed containing contaminated animal byproducts continues to be a source of Salmonella infection, especially in diets containing raw, unpelleted meal.

Humans and animals infected by Salmonella bacteria can be asymptomatic, with clinical signs precipitated by stress. Clinical infections may progress to enteritis, septicemia and variable mortality. A high percentage of survivors become carriers. In humans, acute gastroenteritis can result in sudden onset of abdominal pain, diarrhea, nausea, and fever. Septicemia may sometimes develop in humans, especially in immunocompromised patients.

Fecal and blood culture with selective media have been used to identify Salmonella. However, identification by culture has relatively low sensitivity and is quite slow. Molecular detection of these bacteria, on the other hand, is a highly sensitive and specific technique, and is also more tolerant of suboptimal specimen shipping and handling conditions. This assay is performed on a wide range of sample types, including environmental samples.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Salmonella infection
  • Help ensure animal groups and populations are free of Salmonella
  • Early prevention of spread of these bacteria among a population
  • Minimize human exposure to these bacteria
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

Specimen requirement:

Preferred specimens - Rectal swab, or 0.2 ml feces or bacterial culture, or environmental swab.

Less preferred specimen - 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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