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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

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Stenotrophomonas maltophilia PCR test

wildlife and zoo assay data sheet

Stenotrophomonas maltophilia

Test code:
B0091 - Ultrasensitive qualitative detection of Stenotrophomonas maltophilia by real time PCR.

Stenotrophomonas maltophilia is an aerobic, nonfermentative, gram-negative bacterium. Rare cases of human infections have been described, and are difficult to treat. The bacterium was initially classified as Pseudomonas maltophilia, but subsequently was re-grouped into the genus Xanthomas. In 1993 it was again reclassified into the genus Stenotrophomonas.

S. maltophilia is ubiquitous in aqueous environments, soil and plants, and frequently colonizes respiratory and urinary tracts without adverse effects. In immunocompetent individuals, symptomatic infection with S. maltophilia is uncommon. However, nosocomial infections are possible in immunocompromised individuals. Contamination of biological products with these bacteria during manufacturing processes is also a significant concern.

Treatment of S. maltophilia-infected patients is difficult because this bacterium is resistant to many broad-spectrum antibiotics (including all carbapenems) due to the production of two inducible chromosomal metallo-β-lactamases (designated L1 and L2). Currently many strains of S. maltophilia are still sensitive to co-trimoxazole and ticarcillin, but resistant strains are being reported with increasing frequency.

Laboratory diagnosis of S. maltophilia infection traditionally depended on culture identification, requiring a long incubation time. However, molecular detection by polymerase chain reaction (PCR) is a rapid, sensitive and specific method to detect these bacteria (Cattoir et al., 2010).

Utilities:

  • Confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Stenotrophomonas maltophilia
  • Help ensure that facilities are free of this bacterium
  • Early prevention of spread of S. maltophilia in a facility
  • Minimize human exposure to this bacterium
  • Safety monitoring of biological products

References:
Cattoir, V., Gilibert, A., Le Glaunec, J.M., Launay, N., Bait-Mérabet, L. and Legrand, P. (2010) Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR. Ann. Clin. Microbiol. Antimicrob. 9:21-25.

Specimen requirements:  0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasopharyngeal swab, or 0.2 ml urine, or 0.2 ml feces, or fecal swab, or 0.2 ml fresh, frozen or fixed tissue, or environmental swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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