Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel



Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi



Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii


Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel





Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus




Hepatitis E


Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia


Q fever



Reovirus screen


Rift Valley fever



Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi


Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Trypanosoma evansi PCR test
wildlife and zoo assay data sheet

Surra (Trypanosomiasis)
Etiologic agent: Trypanosoma evansi

Test code:
X0008 - Ultrasensitive qualitative detection of Trypanosoma evansi by real time polymerase chain reaction


Surra is an animal disease occurring in Africa, Asia and Latin America, caused by Trypanosoma evansi. T. evansi belongs to the subgenus Trypanozoon, together with T. equiperdum and T. brucei. The parasite infects various host species and is transmitted by biting flies such as Tabanidae and Stomoxys species, as well as by vampire bats including Desmodus rotondus (Hoare, 1972). Camels and horses are very susceptible to the infection and death can occur within weeks or months. T. evansi infections of cattle and buffalo usually lead to pronounced immunosuppression, resulting in increased susceptibility to other opportunistic diseases such as Pasteurella and anthrax (Stephen, 1986).

Diagnosis of T. evansi infection usually starts with clinical symptoms or the detection of antibodies to T. evansi. Conclusive evidence of T. evansi infection, however, relies on detection of the parasite in the blood or tissue fluids of infected animals. Unfortunately, parasitological techniques cannot always detect ongoing infections as the level of parasitemia is often low and fluctuating, particularly during the chronic stage of the disease (Nantulya, 1990).

Molecular detection of T. evansi using the polymerase chain reaction is a highly sensitive and specific alternative to parasitological tests.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Trypanosoma evansi infection.
  • Help ensure that horse populations are free of T. evansi
  • Early prevention of spread of this parasite
  • Minimize personnel exposure to this parasite
  • Safety monitoring of biological products that derive from horses

Hoare, C.A. (1972) The trypanosomes of mammals. Oxford, Blackwell Scientific Publications 1972, 1-749.
Nantulya, V.M. (1990) Trypanosomiasis in domestic animals: the problems of diagnosis.
Rev Sci Tech 1990, 9:357-367.
Stephen, L.E. (1986) Trypanosomiasis. A veterinary perspective (Edited by: StephenLE). Oxford, Pergamon Press 1986.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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