Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Vesicular stomatitis PCR test
wildlife and zoo assay data sheet

Vesicular stomatitis virus (VSV)

Test code: S0068 - Ultrasensitive qualitative detection of vesicular stomatitis virus by reverse transcription coupled real time polymerase chain reaction. This assay detects and differentiates the New Jersey and Indiana serotypes of the vesicular stomatitis virus.

 

Vesicular stomatitis (VS) is a disease of horses, cattle, swine, sheep, goats and deer caused by related viruses in the genus Vesiculovirus of the family Rhabdoviridae. The viral genome is 11 kb and codes for five main proteins: the nucleocapsid (N), phosphoprotein (P), matrix (M), glycoprotein(G), and polymerase (L). Serologically, VS viruses have been classified into two main serotypes: New Jersey (NJ) and Indiana (IN). In addition to the classic VSV-IN (IN1), two additional subtypes, Cocal virus (VSV-IN2) and Alagoas virus (VSV-IN3), have been described.

VS is prevalent in northern South America, throughout Central America, and at one site in the United States: Ossabaw Island, Georgia. However, outbreaks of VS have been reported in the western United States, northern Mexico, and temperate regions of South America. The disease can cause blisters on an animal's mouth, teats or hooves. Clinical outbreaks of VS in livestock can result in significant economic impact on producers and extensive regulatory responses by governmental agencies, including trade restrictions, market closures, and quarantines. Such measures are needed because of the clinical similarities between VS and foot-and-mouth disease and other foreign vesicular diseases in livestock species.

Utilities:

  • Help confirm the disease causing agent
  • Distinguish between VS and other vesicular diseases including foot-and-mouth
  • Shorten the time required to confirm a clinical diagnosis of VS infection
  • Help ensure that animal herds and populations are free of VS
  • Early prevention of spread of VS
  • Minimize human exposure to the virus

References:
Magnuson, R.J., Triantis, J., Rodriguez, L.L., Perkins, A., Meredith, C.O., Beaty, B., McCluskey, B., and Salman M. (2003) A single-tube multiplex reverse transcription-polymerase chain reaction for detection and differentiation of vesicular stomatitis Indiana 1 and New Jersey viruses in insects. J. Vet. Diagn. Invest. 15:561-567.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or lesion swab or nasopharyngeal swab, or 0.2 ml nasal wash, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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