Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Vibrio PCR test

wildlife and zoo assay data sheet

Vibrio

Test code: B0083 - Ultrasensitive qualitative detection of Vibrio bacteria by real time polymerase chain reaction. This assay detects but does not differentiate V. alginolyticus, V. parahaemolyticus, V. anguillarum, V. cholerae, V. furnissii, V. vulnificus, V. harveyi.

Vibrio is a genus of curved rod-shaped gram negative bacteria that includes several pathogenic species. Most Vibrio bacteria inhabit saltwater, though some species are also found in brackish or fresh water.  They are facultative anaerobes that test positive for oxidase and do not form spores. All members of the genus are motile with polar flagella.

Vibrio bacteria pathogenic to humans include V. cholerae (the causative agent of cholera), V. parahaemolyticus, and V. vulnificus. Infection with V. cholerae is usually a result of drinking contaminated water, whereas infections with V. parahaemolyticus and V. vulnificus are usually caused by eating undercooked contaminated seafood. Although V. parahaemolyticus is the most common noncholera Vibrio species reported to cause illness in humans, V. vulnificus is associated with up to 94% of noncholera Vibrio infection-related deaths. Human infection with these bacteria usually causes gastroenteritis but Vibrio species can also infect open wounds and cause septicemia. Severe infections can be fatal.

Several Vibrio species can cause diseases in a range of animal species.  Marine crustaceans and fish can be carriers of various Vibrio species. Vibrio infections can spread rapidly through aquaculture facilities and can result in significant mortality.

In the past, conventional detection of Vibrio bacteria relied on isolation procedures by growing the bacteria in enrichment broth followed by plating on selective media. The process is laborious and time consuming. Furthermore, the biochemical properties of Vibrio species are similar to those of other bacteria such as Aeromonas species; this has often made unambiguous isolation of Vibrio difficult. On the other hand, detection of Vibrio bacteria by PCR is rapid and sensitive (Maheshwari et al., 2011).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Vibrio
  • Help ensure that animals and facilities are free of Vibrio
  • Early prevention of spread of Vibrio
  • Minimize human exposure to Vibrio
  • Safety monitoring of biological products that derive from susceptible animals

References:
Maheshwari, M., Krishnaiah, N. and Ramana, D.B.V. (2011) Evaluation of polymerase chain reaction for the detection of Vibrio cholerae in contaminants. Annals Biol. Res., 2:212-217.

Specimen requirements: 0.2 ml of blood collected in EDTA (purple top) or ACD (yellow top), or 0.2 ml fresh, frozen or fixed tissue, or wound swab, feces, water sample, bacterial culture, food sample

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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