wildlife and zoo assay data sheet
B0062 - Qualitative ultrasensitive detection of
bacteria by real time polymerase chain reaction.
is a gram-negative bacterium which primarily causes disease in
animals, although humans may also be infected through food
contamination. Infection with this pathogen
can cause tuberculosis-like symptoms, including localized tissue
necrosis and granulomas in the spleen, liver, and lymph node.
occur widely in various host species, including dogs, cats,
horses, cattle, rabbits, deer, rodents, and birds. Documented
cases of occupational exposure to
Y. pseudotuberculosis include butchers working in abattoirs
slaughtering swine. Outbreaks of
in animal facilities have occasionally been reported, including
in primate facilities (Bronson et al., 1972; Iwata et al.,
pseudotuberculosis is very difficult, and serologic tests in
many cases require serial testing on multiple serum samples
taken at several time points. Molecular detection by PCR offers
a rapid, sensitive and specific method for detecting
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Yersinia
Help ensure animal groups and populations are free of
Early prevention of spread of
among a population
Minimize human exposure to
Safety monitoring of biological products and vaccines
that derive from susceptible animals
Bronson, R.T., May, B.D. and Ruebner, B.H. (1972) An Outbreak of
Infection by Yersinia pseudotuberculosis in Nonhuman Primates. Am. J. Pathol. 69:
Iwata, T., Une, Y., Okatani, A.T., Kato, Y., Nakadai,
A., Lee, K., Watanabe, M., Taniguchi, T., Elhelaly, A.E., Hirota,
Y. and Hayashidani, H. (2008) Virulence characteristics of
Yersinia pseudotuberculosis isolated from breeding monkeys in
Japan. Vet Microbiol. 129:404-409.
Rectal swab, or cloacal swab, or 0.2 ml feces, or 0.2 ml fresh,
frozen or fixed tissue, or 0.2 ml whole blood in EDTA (purple
top) or ACD (yellow top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time PCR