Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

For wild rodent infestations, remediation and environmental monitoring, use our Rodent Infestation PCR Panel

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Zoologix also performs rodent and rabbit PCR tests for...

Aspiculuris tetraptera

Bordetella

BXV-1 virus

Campylobacter

Chapparvovirus

Clostridium piliforme

Coccidia

E. coli (enteroinvasive)

Ectromelia

EDIM

Encephalitozoon cuniculi

Encephalomyocarditis

Francisella tularensis

Fur mites

Hantavirus

Helicobacter

Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus

Leptospira

Lymphocytic choriomeningitis virus (LCMV)

Mites

Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse kidney parvovirus (MKV or MKPV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen

Pasteurella

Pinworms

Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit coronavirus

Rabbit fibroma virus

Rabbit hemorrhagic disease virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)

Rotavirus

Salmonella

Sendai virus (SEND)

Seoul virus

Shigella

Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi/ paraluiscuniculi

Tularemia

Tyzzer's disease

Whitewater Arroyo virus

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Whitewater Arroyo virus PCR test

rodent and rabbit assay data sheet

Whitewater Arroyo virus

Test code: S0253 - Ultrasensitive qualitative detection of Whitewater Arroyovirus by reverse transcription coupled real time PCR.

Whitewater Arroyo virus is a single-stranded RNA virus belonging to the Mammavirus genus of family Arenaviridae. This is an emerging virus first reported in woodrats in the Southwest region of North America. Besides woodrats, antibodies against the virus have been reported in other rodents such as Nelson’s pocket mice, northern pygmy mice (Baiomys taylori), northern grasshopper mice (Onychomys leucogaster), oryzomys (Oryzomys spp.), deermice (Megadontomys nelsoni and Peromyscus spp.), harvest mice (Reithrodontomys spp.), cotton rats (Sigmodon spp.), and Merriam’s kangaroo rats.

Woodrats are the major primary host of the virus. Infected woodrats can develop lymphocytic meningitis and perivascular lymphocytic cuffing in the brain. The discovery that WWAV can be recovered from antibody-positive woodrats suggests that WWAV infection can become chronic in these animals. These infected animals are a reservoir host that can continuously spread the virus.

Infection of humans is not common but occurs occasionally. Patients infected with the virus can develop non-specific febrile symptoms, such as fever, headache, muscle pain and acute respiratory distress. Infected patients can have a high mortality rate.

Using serology to track this infection in woodrats is not suitable for most purposes because animals with positive serology could either be active carriers of the virus or may have been previously exposed to the virus and cleared it (Fulhorst et al., 2002). Molecular detection of the virus by PCR can rapidly identify the presence of the virus in a specific and sensitive manner.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of this virus
  • Help ensure that rodent populations and colonies are free of this virus
  • Early prevention of spread of this virus among a population or in a geographic area
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from rodents

References:
Fulhorst, C.F., Milazzo, M.L., Carroll, D.S., Charrel, R.N., Bradley, R.D. (2002) Natural host relationships and genetic diversity of Whitewater Arroyo virus in southern Texas. Am. J. Trop. Med. Hyg. 67:114-118.

Specimen requirements: 1 fecal pellet, or 0.1 ml whole blood in EDTA (purple top) tube, or 0.1 ml fresh or frozen tissue, or throat swab, or 0.1 ml urine

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time polymerase chain reaction

Normal range: Nondetected

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