rodent and rabbit assay data sheet
Mouse cytomegalovirus type 1 and type 2 (MCMV-1 and MCMV-2)
- Ultrasensitive qualitative detection of murine cytomegalovirus
type 1 (MCMV1) by real time PCR.
Ultrasensitive qualitative detection of murine cytomegalovirus
type 2 (MCMV2) by real time PCR.
Ultrasensitive qualitative detection but not differentiation of
murine cytomegalovirus type 1 (MCMV1) and type 2 (MCMV2) by real
and S0164 are
P0029 - Mouse Essentials Panel
Mouse cytomegalovirus (MCMV) is a member of the beta herpes
virus family and is a large, double stranded DNA virus with a
genome size of approx 230 kb. Similarly to rat CMV, two strains
of MCMV have been described: MCMV1, and more recently MCMV2
(Teterinal et al., 2009).
MCMV has broad tissue tropism, and can infect the host’s
epithelial cells such as salivary gland tissue, as well as
macrophages and lymphoid cells. In natural infections, no
clinical symptoms may be detected. The submandibular salivary
gland is the primary site of lesions; other salivary glands are
rarely involved. A substantial percentage of infected mice may
eventually develop latent infections, and the virus may be
transmitted to other mice through biting.
Wild mice remain the reservoir for the virus. Approximately 90%
of wild mice are infected with MCMV. Thus, vivariums should be
equipped with anti-rodent barriers to prevent the mouse colonies
from coming into contact with wild mice.
Both serological and molecular detection methods have been used
to diagnose MCMV infection. However, molecular detection by PCR
is especially useful not only because it is highly sensitive and
specific but also because it can identify mice that are latent
carriers of the virus.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of
Help ensure that vivariums are free of MCMVs
Early prevention of spread of murine cytomegaloviruses among
Minimize personnel exposure to these viruses
Safety monitoring of biological products that derive from
Specimen requirements: Fecal pellet, or
0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml cell culture
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Qualitative real time
polymerase chain reaction
Normal range: Nondetected